Sunday, 11^st September 2005

13:00 - 15:00 Registration. Setting up posters and stands.

15:00 - 15:10  Welcome address. Director of PCB, TT2005 Coordinators.

15:10 - 15:20  Presentation of the meeting. TT2005 Coordinators.

Session I: Vertebrate Non-mammalian transgenic animals 
Co-chairs: Lluis Montoliu (CNB, Madrid) - Nelson Khoo (Umea Univ., Sweden)

15:20 - 15:50
Transgenic Fish: Complementary models to mammalian systems for studying gene function.
Robert N. KELSH
Centre for Regenerative Medicine, Univeristy of Bath, UK

15: 50 - 16:20
Mammalian germ-line transgenesis by transposition, sleeping beauty transposon system. from fish to mice.
Junji TAKEDA
Osaka University, School of Medicine, Osaka, Japan

Session II: genOway Prize for Transgene Technologies 

16:20 - 16:30
Presentation of genOway Prize for Transgene Technologies
Kader THIAM
genOway, Lyon, France

16:30 - 17:15
Talk given by the TT2005 genOway Prize Winner
Andras NAGY
Mount Sinai Hospital, Samuel Lunenfeld Research Instiute, Toronto, Canada

17:15 ? 17:45  Coffee break

Session III: New technologies in Transgenesis 
Co-chairs: Lluis Montoliu (CNB, Madrid) -Nelson Khoo (Umea Univ., Sweden)

17:45 - 18:15
The ET-cloning system and beyond.
A. Francis STEWART
Technische Universität Dresden, BioInnovationZentrum, Dresden, Germany

18:15 - 18:45
Use of lentiviral-type vectors in transgenic animals.
Bruce WHITELAW
Roslin Institute (Edinburgh), Roslin, UK

18:45 - 19:15
RNAi in expression vectors.
Michaela SCHERR
Medizinische Hochschule, Hannover, Germany

19:15 - 19:45
Monitoring CRE recombination at cellular level, FLEX transgenesis.
Norbert B. GHYSELINCK
Institut de Génétique et de Biologie Molléculaire et Cellulaire (IGBMC), Illkirch (Strasbourg), France

20:00 - 21:30  Get-together Buffet offered to participants, visit posters and stands.

21:30    Barcelona sightseeing at night

Monday 12^nd September 2005

Session IV: Generation and Breeding of Transgenic and Knockout Mice 
Chair: Johannes Wilbertz (Karolinska I., Sweden)

9:00-9:30
Choice of Strains/Strain Genetic-Differences/Modifiers
David WOLFER
Institut of Anatomy, University of Zürich, Zürich, Switzerland

9:30-10:00
Welfare and characterization of genetically modified mice
Rikke THON
Harlan, Denmark

10:00-10:30
Mouse Colony Management (Databases)
Marie-Noëlle MARTINEZ
Charles River Laboratories, France

10:30-11:00
Refinement techniques in Transgenesis
Dominic J. WELLS
Department of Cellular and Molecular Neuroscience, Imperial College Faculty of Medicine, London, UK

11:00 - 11:30  Coffee-Break

Session V: Funtional genomics. Using mice/transgenic mice to understand Complex Mammalian Genomes 
Chair: Mariona Arbones (CRG, Barcelona)

11:30-12:00
The Eumorphia Project
Steve BROWN
MRC Mammalian Research Unit, Harwell, UK

12:00-12:30
European Mouse Mutant Archive (EMMA)
Martin HRABÉ DE ANGELIS
GSF National Research Center for Environment and Health, Institute of Experimental Genetics, Neuherberg, Germany

12:30-13:00
Public Gene Trap Initiative
Harald VON MELCHNER
Laboratory for Molecular Hematology, University of Frankfurt Medical School, Frankfurt am Main, Germany

13:30-15:30  Lunch, Visiting Posters and Stands

Session VI: Running a Transgenic Unit. Round-table discussion 
Co-chairs: Johannes Wilbertz (Karolinska I., Sweden) / Belen Pintado (INIA, Madrid, Spain)

15:30-17:30
Frank ZIMMERMANN
Biotechnologie Labor (Transgenic Unit), University of Heidelberg, Heidelberg, Germany

Francina LANGA
Centre d'Ingénierie Génétique Murine (CIGM), Institut Pasteur, Paris, France

Sagrario ORTEGA
Transgenic Unit, Biotechnology Program, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

Thom SAUNDERS
Transgenic Animal Model Core, University of Michigan, Ann Arbor, Michigan, USA

Shirley PEASE
Transgenic and Knockout Core Facility, California Institute of Technology (Caltech), Pasadena, California, USA

Session VII: Non-invasive methods: Imaging 
Chair: Sagrario Ortega (CNIO, Madrid)

17:30-18:00
3D imaging
James SHARPE
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK

18:00-18:30
In vivo imaging
Marc JANIER
ANIMAGE ? CREATIS / Hôpital Edouard Herriot, Lyon, France

18:30-19:00
Molecular Imaging: Structural and functional techniques
Manuel DESCO
Laboratorio de Imagen. Medicina Experimental. Hospital General Universitario "Gregorio Marañón", Madrid, Spain
 

Session VIII: Founding an European/International Transgenic Tech. Society? 
19:00-20:00  Round-table/Debate: Lluis Montoliu / Johannes Wilbertz / Nelson Khoo

21:30    TT2005 Dinner and Live Music

Tuesday 13^rd September 2005

Session IX: Phenotypic Analysis of Genetically Modified Mice 
Co-chairs: Anna Pujol (CBATEG, UAB, Barcelona), Belen Pintado (INIA, Madrid)

9:00-9:20
Clinical physiology of genetically modified mice
Raymond ROMAND
Institut de Génétique et de Biologie Molléculaire et Cellulaire (IGBMC), Illkirch (Strasbourg), France

9:25-9:45
Pathology of genetically modified mice
Pierre DUBUS
Department of Histology and Molecular Pathology. University and Hospital of Bordeaux. Bordeaux, France

9:50-10:10
SHIRPA/Behavioral analysis of genetically modified mice
Mara DIERSSEN
Genes and Disease Program, Genomic Regulation Center, Barcelona, Spain

10:15-10:35
Breeding schemes: Speed congenics / Standard congenics
Jean-Louis GUÉNET
Institut Pasteur, Paris, France

10:40-11:10
Phenotypic analysis of Metabolic Diseases: diabetes
Fàtima BOSCH
CBATEG, Barcelona, Spain

11:15-11:45  Coffee Break, Visiting Posters and Stands

Session X: Hot topics in mouse genetics and development 
Co-Chairs: Mariona Arbones (CRG, Barcelona), Lluis Montoliu (CNB, Madrid)

11:45-12:15
Genomic Imprinting
Gavin KELSEY
The Babraham Institute, Cambridge, UK

12:15-12:45
Cell cycle and mouse genetics
Marcos MALUMBRES
Molecular Oncology Program, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

12:45-13:15
Mouse genetics and CNS disorders
Xavier ESTIVILL
Genes and Disease Program, Genomic Regulation Center, Barcelona, Spain

13:15-13:30
A new method to produce mice fully derived from embyonic stem cells
David VALENZUELA
Regeneron Pharmaceuticals, Tarrytown, NY, USA

13:35-13:40
Concluding Remarks
Lluís Montoliu

13:40-13:45
Next TT meeting in Brisbane (Australia)
Elizabeth Williams
Transgenic Animal Service of Queensland. Institute for Molecular Biosciences.
University of Queensland, Australia.

14:00-16:00 Lunch, Visiting Posters and Stands

Course on Basic Transgenic Techniques 

16:00-16:30
Construct Design for transgenes and gene targeting experiments
Alfonso GUTIÉRREZ-ADÁN
Departamento de Reproducción Animal, INIA, Madrid, Spain

16:30-17:00
ES cell culturing (cells, methods, problems)
Sagrario ORTEGA
Transgenic Unit, Biotechnology Program, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain

17:00-17:30
What strains are the best for transgenic/knock-out experiments?
Tom FIELDER
Transgenic Mouse Facility, UC-Irvine, Irvine, California, USA

17:30-18:00
Microbial agent impact on animal experiments
Jose Manuel SÁNCHEZ-MORGADO
The National Institute for Medical Research (MRC-NIMR), London, UK

18:00-18:30
Surgery (post- and pre-operation case) and narcotics
Ignacio ÁLVAREZ
Hospital Universitario La Paz, Cirugía Experimental, Madrid, Spain