TILLer is a TILLING service aiming to identify point mutations induced by EMS in Arabidopsis gene sequences ordered by users. For that, this facility has developed a permanent collection of highly EMS mutagenised M3 families in Arabidopsis Landsberg erecta glabrous1 (Ler gl1) background. Mutant families in genes of interest are selected by reverse genetic analysis using a TILLING assay based on digestion with the CEL 1 endonuclease (Colbert et al., 2001, Plant Physiology 126:480-484; Greene et al., 2003, Genetics 164:731-740). Furthermore, TILLer provides the mutant seeds to users.


TILLer Service has been published as Martin.B et al, 2009, BMC Plant Biology 9:147