The SEVA platform is a coherent resource of molecular tools subjected to a concise, minimalist, and standardized format and nomenclature, fully compatible with old and new cloning protocols and DNA assembly methods. Simple assembly rules allow the generation of a huge collection of vectors that cover virtually all genetic and metabolic engineering necessities of P. putida KT2440 and other Gram-negative bacteria: genome editing (i.e. deletions and insertions), heterologous gene expression, protein secretion, metabolic pathways, logic circuits, biosensors with optical readouts, among many others. With these tools in hand, we are developing and adapting to P. putida a large number of Synthetic Biology resources, both computational (e.g. the Synthetic Biology Open Language, CELLO in vivo programming) and experimental (MAGE, CRISPR/Cas9, proteomic and transcriptional switches). These will allow setting the catalytic properties of P. putida to be deployed following given specifications of time, space and environmental conditions.
People involved: Esteban Martínez-García, Angel Goñi, Tomás Aparicio, Belén Calles, Sofía Fraile
– pSEVA collection Webpage