Tomás Aparicio

I got a PhD in Biology under the supervisión of Dr. Perera (Faculty of Biology-UCM, 2000), where I studied the biology of a Thiobacillus plasmid. After that my profesional track included experience in a biotech company (PharmaMar S.A.-Madrid, 2001-2004), technical work in the Unit of Genomics of Scientific Park of Madrid (PCM, 2004-2009), a post-doc period in the CBMSO (Madrid, 2010) under the supervisión of Dr. García-Ballesta and also teaching activities as Associate Professor in UCM and UFV universites. Although involved in different research fields along my career, I have been mainly focused in Microbiology and Bacterial Genetic Engenieering.

In Victor´s Lab (CNB-Madrid, 2013-), I have a technical position aimed to give support for general laboratory activities, focusing on P. putida KT2440 strain engineering, collections maintenance and pSEVA platform development. I am also involved in the implementation of novel technical procedures to keep the state-of-the art, specially in the area of DNA assembly methods such as Isothermal Assembly, USER, BASIC, Yeast Assembly, etc. Besides that, I run a number of specific projects such as the development of secretion systems in P. putida KT2440, sensitization of this bacteria to lambda phage and construction of pSEVA shuttle vectors.


Figure 1. Assembly of a broad host pSEVA-shuttle for Gram+/Gram- bacteria. (a) Functional elements of pSEVA-shuttle (namely: broad host range Gram+ and Gram- replicons, a Cm resistance marker and the oriT), were amplified by PCR. (b) PCR fragments were assembled by USER reaction and transformed in E. coli. (c) Plasmid structure was confirmed by sequencing. (d) pSEVA-shuttle vector was transformed in Lactococcus lactis, replicating succesfully in this Gram+ host.