Alberto Sánchez-Pascuala

Hello! As a kid I was fascinated by the term “molecular biology”. I remember well the first time that I watched the film “Jurassic Park” and something inside me said… I must be scientist!
In order to achieve my goals I started my undergraduate studies at the University of Alcalá (Madrid – Spain), where I obtained my degree in Biology with specialization in Molecular Biology and Biotechnology. The summer research visit that I made in this lab and the last year of my degree showed me an amazing field, the microbiology. This interest culminated with my Master degree in Microbiology at the Autonomous University of Madrid (Madrid – Spain), the starting point of my current challenge, my PhD training.

Pseudomonas putida KT2440 is an appealing bacterium in order to implement in biotechnological processes; this is due to a remarkable metabolic versatility and amenability to genetic manipulation. Nevertheless, this microorganism often shows a non-optimal energetic yield that limits their efficiency under operation conditions. To get over this challenge, we have focused our attention on the study of the glycolysis of Pseudomonas, which is hardly efficient on ATP acquisition terms.

In order to overcome this state of affairs, and elucidate at the same time the functioning of the central metabolic pathways in P. putida, we employ a set of genetic tool to edit directly the chromosome of this microorganism. This approach is based on the deletion of different glycolytic genes to produce a streamlined chassis where to introduce a pool of metabolic reactions to allow an efficient heterologous glycolytic pathway.
Taking into account the mentioned guidelines, our work pursues to construct a domesticated P. putida strain in terms of metabolic capabilities, thereby generating a robust cell factory (heeding its energetic yield and oxidative stress resistance) to implement in different biotechnological purposes.

albertopascuala

Implementation of Embden–Meyerhof–Parnas (EMP) pathway in P. putida SC (P. putida KT2440 with deletions in different glycolytic genes). The growth phenotype in glucose is restored by complementation with pGBI plasmid, which expresses some EMP genes from E. coli MG1655 in a pSEVA224 backbone.