Publication List

328.  Kim, J., Pérez-Pantoja, D., Silva-Rocha, R., Oliveros, J.C. and de Lorenzo, V. (2015) High resolution analysis of the m-xylene/toluene biodegradation subtranscriptome of Pseudomonas putida mt-2. Env Microbiol (In Press).

327.  Arce-Rodríguez, A., Calles, B., Nikel, P-I. and de Lorenzo, V. (2015) The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas. Putida. Env Microbiol (In Press).

326.  Nikel, P.I., Chavarría, M., Führer, T., Sauer, U. and de Lorenzo V. (2015) Pseudomonas putida KT2440 metabolizes glucose through a cycle formed by enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and pentose phosphate pathways. J Biol Chem (In Press)

325.  Wierckxn, N., Prieto, A., Pomposiello, P., de Lorenzo, V., O’Connor, K., Blank, L. M. (2015) Plastic waste as novel substrate for Industrial Biotechnology. Microb Biotechnol (In Press)

324.  Deuschle, M., Limbrunner, S., Rother, D., Wahler, S., Chavarría, M., de Lorenzo, V., Kremling, A., and Pflüger-Grau, K. (2015). Interplay of the PtsN (EIIANtr) protein of Pseudomonas putida with its target sensor kinase KdpD. Env Microbiol Rep (In press)

323.  Zobel, S., Benedetti, I., Eisenbach, L., de Lorenzo, V., Wierckx, N. and Blank, L. (2015) A Tn7-based device for calibrated heterologous gene expression in Pseudomonas putida. ACS Synth Biol (In press)

322.  Aparicio, Y., de Lorenzo, V. and Martínez-García, E. (2015) Broadening the SEVA plasmid repertoire to facilitate genomic editing of Gram-negative bacteria McGenity et al. (eds.), Springer-Verlag. Hydrocarbon and Lipid Microbiology Protocols. DOI 10.1007/8623_2015_102 pubmed

321.  Kim, J and de Lorenzo, V. (2015) Detection of specific mRNA molecules in hydrocarbon-degrading bacteria. McGenity et al. (eds.), Springer-Verlag. Hydrocarbon and Lipid Microbiology Protocols. DOI 10.1007/8623_2015_100. pubmed

320.  Calles, B., de Lorenzo V. (2015) Knock-In-Leave-Behind (KILB): genetic grafting. of protease-cleaving sequences into permissive sites of proteins with a Tn5-based transposition system McGenity et al. (eds.), Springer-Verlag. Hydrocarbon and Lipid Microbiology Protocols. DOI 10.1007/8623_2015_114,

319.  Kim, J and de Lorenzo, V. (2015) Deep sequencing to study microbial transcriptomic responses to hydrocarbon degradation/production/stress. McGenity et al. (eds.), Springer-Verlag. Hydrocarbon and Lipid Microbiology Protocols. DOI 10.1007/8623_2015_101pubmed

318.  de Lorenzo, V. (2015). Designing bacteria for the environment: from trial and error to earnest engineering. In Biosafety and the Environmental uses of Microorganisms, OEDC Publishing, pp 103-114

317.  Jimenez, J.I., Fraile, S., Zafra, O. And de Lorenzo, V. (2015) Phenotypic knockouts of selected metabolic pathways by targeting enzymes with camel-derived nanobodies (VHHs). Metab. Eng. 30: 40-48.pubmed

316.  Pazos, F. and de Lorenzo, V. (2015) Biodegradation Prediction Tools. In T.J. McGenity et al. (eds.), Springer-Verlag. Hydrocarbon and Lipid Microbiology Protocols. DOI 10.1007/8623_2015_87

315.  Nikel, P.I., Romero-Campero, J, Zeidman, JA, Goñi-Moreno, A. and de Lorenzo V. (2015) The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor. mBio 6 (2).

314.  Páez-Espino, A. D., Chavarría, M. and de Lorenzo, V. (2015) The two paralog phoN (phosphinothricin acetyl transferase) genes of Pseudomonas putida encode functionally different proteins. Env Microbiol (In Press).

313.  Lieder, S., Nikel, P.I., de Lorenzo, V. and Takors, R. (2015) Genome reduction boosts heterologous gene expression in Pseudomonas putida. Microb Cell Fact 14: 23.

312. de las Heras, A., Martínez-García, E., Domingo Sananes M.R and de Lorenzo, V. (2015) Widening functional boundaries of the σ54 promoter Pu of Pseudomonas putida by defeating extant physiological constraints. Mol BioSys 11: 734-42

311.  Martínez-García, E., Benedetti, I., Hueso, A, and de Lorenzo, V. (2015) Mining environmental plasmids for Synthetic Biology parts and devices. Microbiol Spectrum 3(1

310. Martínez-García, E., Aparicio, T., Goñi-Moreno, A., Fraile, S. and de Lorenzo, V. (2014) SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities. Nucl Acids Res.pubmed Print

309. Martínez-García E., Nikel P.I., Aparicio, T. and de Lorenzo, V. (2014) Pseudomonas 2.0: Genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expresión. Microb Cell Fact. pubmed  Print

308. de Lorenzo, V. (2014) It’s the metabolism, stupid! Environ Microbiol (In Press)

307. Martínez-García, E., Aparicio, T., de Lorenzo, V. Nikel, P.I. (2014) New transposon tools tailored formetabolic engineering of Gram-negative microbial cell factories. Front Bioeng Biotechnol. pubmed

306. de Lorenzo, V. (2014). Chassis organism from Corynebacterium glutamicum: The way towards biotechnological domestication of Corynebacteria. Biotechnol J. pubmed

305. de Lorenzo, V. (2014) Pseudomonas aeruginosa: the making of a pathogen. Environ Microbiol. pubmed

304. Porcar, M, Danchin, A., de Lorenzo, V. (2014). Confidence, tolerance and allowance in biological engineering: The nuts and bolts of living things. BioEssays. pubmed

303. de Lorenzo, V., Sekowska, A. and Danchin, A . (2014) Chemical reactivity drives spatiotemporal organization of bacterial metabolism. FEMS Microbiol Revs. pubmed

302. de Lorenzo, V. (2014). Synthetic Biology: Engineering to the conquest of biological complexity (in spanish). Arbor (In Press).

301. Chen J, Sun GX, Wang XX, de Lorenzo V, Rosen BP, Zhu YG (2014) Volatilization of arsenic from polluted soil by Pseudomonas putida engineered for expression of the arsM arsenic(III) S-adenosine methyltransferase gene. Env Sci Technol 48: 10337-10344. pubmed

300. Pflüger-Grau, K. and de Lorenzo, V. (2014) From the phosphoenolpyruvate phosphotransferase system (PTS) to selfish metabolism: a story retraced in Pseudomonas putida. FEMS Microbiol Lett 356: 144-153 pubmed

299. Martínez-García, E., Jatsenko, T., Kivisaar, M. and de Lorenzo, V. (2014) Freeing Pseudomonas putida KT2440 of its proviral load strengthens endurance to environmental stresses. Env Microbiol (In Press)

298. Chavarría, M., Durante-Rodríguez, G., Krell, T., Brezovsky, J., Damborsky, J. and de Lorenzo, V. (2014) Fructose 1-Phosphate is the one and only physiological effector of the Cra (FruR) regulator of Pseudomonas putida. FEBS Open Bio 4: 377–386 pubmed

297. Páez-Espino, A.D, Durante-Rodríguez, G. and de Lorenzo, V. (2014) Functional coexistence of twin arsenic resistance systems in Pseudomonas putida KT2440. Env. Microbiol. (In Press). pubmed

296. Nikel, P.I. Martínez-García, E. and de Lorenzo, V. (2014) Biotechnological domestication of pseudomonads using synthetic biology. Nature Microbiol Revs. 12: 368-379

295. Nikel, P.I. and de Lorenzo, V. (2014) Robustness of Pseudomonas putida KT2440 as a host for ethanol biosynthesis. New Biotechnol 31: 562-571.

294. Pérez-Pantoja, D., Kim, J., Silva-Rocha, R and de Lorenzo, V. (2014) The differential response of the Pben promoter of Pseudomonas putida mt-2 to BenR and XylS prevents metabolic conflicts in m-xylene biodegradation. Environ Microbiol (In Press)

293. Silva-Rocha, R. and de Lorenzo V. (2014) The pWW0 plasmid imposes a stochastic expression regime to the chromosomal ortho pathway for benzoate metabolism in Pseudomonas putida. FEMS Microbiol Lett 356: 176-183.

292. Timmis K, de Lorenzo V, Verstraete W, Garcia JL, Ramos JL, Santos H, Economidis I, Nogales B, Timmis JK, Fonseca C, Pruzzo C, Karagouni A, Panopoulos N, Dixon B. (2014) Pipelines for New Chemicals: a strategy to create new value chains and stimulate innovation-based economic revival in Southern European countries. Environ Microbiol 16: 9-18.

291. Jiménez, J.I., Perez-Pantoja, D., Chavarría, M., Díaz, E., de Lorenzo, V. (2014) A second chromosomal copy of the catA gene endows Pseudomonas putida mt-2 with an enzymatic safety valve for excess of catechol. Env Microbiol 16: 1767-1778.

290. Nikel, P.I., Silva-Rocha, R., Benedetti, I. and de Lorenzo, V. (2014) The private life of environmental bacteria: pollutant biodegradation at the single cell level. Env Microbiol 16: 628-642

289. de Lorenzo, V. (2014) From the selfish gene to selfish metabolism: revisiting the central dogma. BioEssays 36: 226–235

288. Martínez-García, E., Nikel, P.I., Chavarría M. and de Lorenzo V. (2013). The metabolic cost of flagellar motion in Pseudomonas putida KT2440. Env. Microbiol. 16: 291-303.

287. Silva-Rocha, R., Simao Nina de Azevedo, Carepo M., Lopes de Souza, R. Silva A., de Lorenzo V., Cruz-Schneider (2013) Vestigialization of arsenic resistance phenotypes/genotypes in Chromobacterium violaceum strains thriving in pristine Brazilian sites. Biocatal Biotrans. 31: 281-291

286. Billerbeck, S., Calles, B., Müller C.L., de Lorenzo, V., Panke, S. (2013) Towards functional orthogonalization of protein complexes: Individualization of GroEL monomers leads to distinct quasi-homogeneous single rings. Chem BioChem 14: 2310-2321.

285. Calles, B. and de Lorenzo, V. (2013) Expanding the Boolean logic of the prokaryotic transcription factor XylR by functionalization of permissive sites with a protease-target sequence. ACS Synthetic Biology 2: 594–603

284. Kim, J., Oliveros, J.C., Nikel, P., de Lorenzo, V. and Silva-Rocha, R. (2013) Transcriptomic fingerprinting of Pseudomonas putida under alternative physiological regimes. Env Microbiol Rep 5: 883-891

283. Nikel, P.I., Kim, J., de Lorenzo, V. (2013) Metabolic and regulatory rearrangements underlying glycerol metabolism in Pseudomonas putida KT2440. Env Microbiol 16: 239-54.

282. Silva-Rocha, R. and de Lorenzo, V. (2013) Engineering multicellular logic in bacteria with metabolic wires. ACS Synthetic Biology 3: 204-209.

281. Pérez-Pantoja, D., Nikel P.I., Chavarría M. and de Lorenzo, V. (2013) Endogenous stress caused by faulty oxidation reactions fosters evolution of 2,4-dinitrotoluene-degrading bacteria. PLoS Genetics 9:e1003764

280. Nikel, P.I. Chavarría, M., Martínez-García, E., Taylor, A.C. and de Lorenzo, V. (2013) Accumulation of inorganic polyphosphate enables stress endurance and catalytic vigour in Pseudomonas putida KT2440. Microb Cell Fact 12: 50.

279. Chen, J., Qin, J., Zhu, Y., de Lorenzo, V. and Rosen, B. (2013) Engineering the soil bacterium Pseudomonas putida for arsenic methylation. App Env Microbiol 79: 4493-4495

278. Fraile, S., Jiménez, J.I., Gutiérrez, C. de Lorenzo, V. (2013) NanoPad: an integrated platform for bacterial production of camel nanobodies aimed at detecting environmental biomarkers. Proteomics 13: 2766-75

277. Durante-Rodríguez, G., de Lorenzo, V. and Martínez-García, E. (2014) The Standard European Vector Architecture (SEVA) plasmid toolkit. Methods Mol. Biol. 1149: 469-478

276. Silva-Rocha, R. and de Lorenzo, V. (2014) Chromosomal integration of transcriptional fusions. Methods Mol. Biol. 1149: 479-489

275. Benedetti, I and de Lorenzo, V. (2014) Promoter fusions with optical outputs in individual cells and in populations. Methods Mol. Biol. 1149: 579-590

274. de Lorenzo, V., Pieper, D. and Ramos, J.L. (2014) From the test tube to the Environment –and back. Environ Microbiol 15: 6–11

273. Nikel, P., Pérez-Pantoja, D. and de Lorenzo, V. (2013) Why are chlorinated pollutants so difficult to degrade aerobically? Redox stress limits 1,3-dichloprop-1-ene metabolism by Pseudomonas pavonaceae. Phil Trans B Roy Soc 368: 20120377

272. Chavarría, M., Nikel, P.I, Pérez-Pantoja, D. and de Lorenzo, V. (2013) The Entner-Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress. Environ Microbiol 15: 1772-1785

271. Silva-Rocha, R., Pérez-Pantoja, D. and de Lorenzo, V. (2013) Decoding the genetic networks of environmental bacteria: regulatory moonlighting of the TOL system of Pseudomonas putida mt-2. ISME J 7: 229-322

270. Silva-Rocha, R, Chavarría, M., Kleijn, R., Sauer, W. and de Lorenzo, V. (2013) The IHF regulon of exponentially growing Pseudomonas putida cells. Env Microbiol 15: 49-63

269. Chavarría, M., Fuhrer, T., Sauer, W., Pflüger-Grau, K. and de Lorenzo, V. (2013) Cra regulates the cross talk between the two branches of the phosphoenolpyruvate:phosphotransferase system of Pseudomonas putida. Env Microbiol 15: 121-132

268. Benedetti, I., de Lorenzo, V. and Silva-Rocha, R (2012) Quantitative, non-disruptive monitoring of transcription in single cells with a broad-host range GFP-luxCDABE dual reporter system. PLoS One 7(12):e52000.

267. Kremling, A., Pflüger-Grau, K., Chavarría, M. Puchalka, J., dos Santos, V. M. and de Lorenzo, V (2012) Modeling and analysis of flux distributions in the two branches of the phosphotransferase system in Pseudomonas putida. BMC Systems Biology 6:149.

266. Silva-Rocha, R., Martínez-García, E., Calles, B., Chavarría, M., Arce-Rodríguez, A., de las Heras, A., Páez-Espino, D., Durante-Rodríguez, G., Kim, J., Nikel, P.I., Platero, R. and de Lorenzo, V. (2012) The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucl Acids Res 41: D666-D675.

265. Silva-Rocha, R and de Lorenzo, V. (2012) Bacteria as computers: formalizing transcriptional regulatory networks by way of digital logic circuits. Int J Unc Comp 8: 517-528

264. de Lorenzo, V. (2012) Towards a global Biotechnology scenario. Curr Op Biotech 23: 827-829.

263. Silva-Rocha, R. and de Lorenzo, V. (2012) The TOL network of Pseudomonas putida mt-2 processes multiple environmental inputs into a narrow response space. Env Microbiol 15: 271-286.

262. Nikel, P. and and de Lorenzo, V. (2012) Engineering an anaerobic metabolic regime in Pseudomonas putida KT2440 for the anoxic biodegradation of 1,3-dichloroprop-1-ene. Metab. Eng. 15: 98-112.

261. de las Heras, A., Fraile S. and de Lorenzo, V. (2012) Increasing signal specificity of the TOL network of Pseudomonas putida mt-2 by rewiring the connectivity of the master regulator XylR. PLoS Genetics 8: e1002963

260. Silva-Rocha, R. and de Lorenzo, V. (2012) Stochasticity of TOL plasmid catabolic promoters sets a bimodal expression regime in Pseudomonas putida mt-2 exposed to m-xylene. Mol. Microbiol. 86: 199-211

259. Nikel, P. and de Lorenzo, V. (2012). Implantation of unmarked regulatory and metabolic modules in Gram-negative bacteria with specialised mini-transposon delivery vectors. J. Biotechnol 163: 143-154

258. Silva-Rocha, R and de Lorenzo, V. (2012) Broadening signal specificity of prokaryotic promoters by modifying cis-regulatory elements associated with a single transcription factor. Mol BioSys 8: 1950-1957.

257. Silva-Rocha, R and de Lorenzo, V. (2012) A GFP-lacZ bicistronic reporter system for promoter analysis in environmental Gram-negative bacteria. PLoS One 7(4): e34675.

256. Leprince, A., de Lorenzo, V., Völler, P., van Passel, M.W.J, and Martins dos Santos, V. (2012) Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida. Environ Microbiol 14: 1444-1453.

255. Tamames, J., de la Peña, S. and and de Lorenzo, V. (2012). COVER: A priori estimation of coverage for metagenomic sequencing. Environ Microbiol Rep 4: 335-341.

254. Schmidt, M. and de Lorenzo, V. (2012). Synthetic constructs in/for the environment: Managing the interplay between natural and engineered Biology. FEBS Lett 586: 2199-206.

253. Chavarría, M., Kleijn, R.J., Sauer, U., Pflüger-Grau, K., and de Lorenzo, V. (2012) Regulatory tasks of the phosphoenolpyruvate-phosphotransferase system (PTS) of Pseudomonas putida in Central Carbon Metabolism. mBio 3(2): e00028-12.

252. Jurado, P., Fernandez, L.A. and de Lorenzo, V. (2012). Production and characterization of a recombinant single-chain antibody (scFv) for tracing the sigma 54 factor of Pseudomonas putida. J. Biotechnol. 160: 33-41

251. Silva-Rocha R., Tamames J., and de Lorenzo V. (2012) The logic of decision-making in environmental bacteria. In E. Katz (Ed.) Molecular and Biomolecular Information Processing: From Logic Systems to Sensors and Actuators. Wiley-VCH, pp 279-302.

250. Leprince, A., Janus, D., de Lorenzo, V., dos Santos V. M. (2012) Streamlining of a Pseudomonas putida genome using a combinatorial deletion method based on mini-transposon insertion and the Flp-FRT recombination system. Methods Mol. Biol. 813: 249-266.

249. Silva-Rocha, R., de Jong, H., Tamames, J. and de Lorenzo, V. (2011) The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene BMC Systems Biology 5: 191.

248. Arce-Rodríguez, A., Durante-Rodríguez, G., Platero, R., Krell, T., Calles, B. and de Lorenzo V. (2011). The Crp regulator of Pseudomonas putida: evidence of an unusually high affinity for its physiological effector, cAMP. Env Microbiol. 14: 702-713.

247. Valls, M., Silva-Rocha, R., Cases, I., Muñoz, A., and de Lorenzo, V. (2011) Functional analysis of the integration host factor site of the 54 Pu promoter of Pseudomonas putida by in vivo UV imprinting. Mol Microbiol 82: 591-601

246. de las Heras, A., Chavarría, M. and de Lorenzo V (2011). Association of dnt genes of Burkholderia sp. DNT with the substrate-blind regulator DntR draws the evolutionary itinerary of 2,4-dinitrotoluene biodegradation. Mol Microbiol 82: 287-299

245. Silva-Rocha, R. and de Lorenzo, V. (2011) A composite feed forward loop I4-FFL involving IHF and Crc stabilizes expression of the XylR regulator of Pseudomonas putida mt-2 from growth phase perturbations. Mol BioSys 7: 2982-2990

244. Martínez-García, E. and de Lorenzo, V. (2011) Engineering multiple genomic deletions in Gram-negative bacteria: Analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440. Env Microbiol 13: 2702-2716.

243. Silva-Rocha, R. and de Lorenzo, V. (2011) Implementing an OR-NOT (ORN) logic gate with components the SOS regulatory network of Escherichia coli. Mol BioSys 7: 2389-96.

242. Porcar, M., Danchin, A., de Lorenzo, V., Martins dos Santos, V., Krasnogor, N., Rasmussen, S, and Moya A. (2011) The ten grand challenges of synthetic life. Sys Synth Biol 5: 1-9.

241. Koutinas, M., Kiparissides, A., Lam, M.C., Silva-Rocha, R., Godinho, M. de Lorenzo, V., Martins dos Santos, M.V., Pistikopoulos, E.N., Mantalaris, A. (2011) Improving the prediction of Pseudomonas putida mt-2 growth kinetics with the use of a gene expression regulation model of the TOL plasmid. Biochem Eng J 55: 108–118

240. Martínez-García, E. and de Lorenzo, V. (2011) Transposon-based and plasmid-based genetic tools for editing genomes of Gram-negative bacteria. Methods Mol. Biol. 813: 267-283.

239. de las Heras A. and de Lorenzo, V. (2012) Engineering a whole-cell biosensor with no antibiotic markers for monitoring aromatic compounds in the environment. Methods Mol. Biol. 834: 261-281.

238. Martínez-García, E., Calles, B., Arévalo-Rodríguez, M. and de Lorenzo, V. (2011) pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. BMC Microbiology 11: 38

237. Koutinas, M., Kiparissides, A., Silva-Rocha, R., Lam, M.C., dos Santos, M.V., de Lorenzo, V., Pistikopoulos, E.N., Mantalaris, A. (2011). Linking genes to microbial growth kinetics -an integrated biochemical systems engineering approach. Metabol Engineer 13: 401-413.

236. Silva-Rocha R, Tamames J, dos Santos VM and de Lorenzo V (2011) The logicome of environmental bacteria: merging catabolic and regulatory events with Boolean formalisms. Environ Microbiol 13: 2389-2402.

235. Pflüger-Grau, K, Chavarría, M. and de Lorenzo, V. (2011) The interplay of the EIIANtr component of the nitrogen-related phosphotransferase system (PTSNtr) of Pseudomonas putida with pyruvate dehydrogenase. Bioch. Biophys. Acta 1810: 995-1005.

234. Chavarría, M., Santiago, C., Platero, R., Krell, T., Casasnovas, J.M. and de Lorenzo, V. (2011) Fructose 1-Phosphate is the preferred effector of the metabolic regulator Cra of Pseudomonas putida. J. Biol. Chem. 286: 9351-9359.

233. de las Heras, A. and de Lorenzo, V. (2011). Cooperative amino acid changes shift the response of the 54-dependent regulator XylR from natural m-xylene towards xenobiotic 2,4-dinitrotoluene. Mol Microbiol 79: 1248-1259.

232. de las Heras, A. and de Lorenzo, V. (2011). In situ detection of aromatic compounds with biosensor Pseudomonas putida cells preserved and delivered to soil in water-soluble gelatin capsules. Analyt. Bioanalyt. Chem. 400: 1093-1104.

231. Zafra, O., Fraile, S, Gutiérrez, C., Haro, A., Páez-Espino, D., Jiménez, J.I. and de Lorenzo, V. (2011) Monitoring biodegradative enzymes with nanobodies raised in Camelus dromedarius with mixtures of catabolic proteins. Env Microbiol 13: 960-974.

230. de Lorenzo, V. (2011) Genes that move the window of viability of life: lessons from bacteria thriving at the cold extreme. BioEssays 33: 38-42.

229. de Lorenzo, V. (2011). Beware of metaphors: chasses and orthogonality in Synthetic Biology. BioEngineered Bugs 2: 1-5

228. Milanesio, P., Arce, A., Muñoz, A., Calles, B. and de Lorenzo, V. (2011) Regulatory exaptation of the catabolite repression protein (Crp)-cAMP system in Pseudomonas putida. Environ Microbiol 13: 324-39.

227. Gilbert, D., Jaramillo, A., Krasnogor, N., de Lorenzo V. (2010) Synthetic Biology gains momentum in Europe. Syst Synth Biol. 4: 145-147.

226. de Lorenzo, V. (2010) Synthetic Biology: something old, something new. BioEssays 32: 267-270.

225. de Lorenzo, V. (2010). Beautiful is not (necessarily) right: Overcoming the Phryne’s trial syndrome. BioEssays 32: 1011

224. de Lorenzo, V. (2010). Environmental biosafety in the age of Synthetic Biology: Do we really need a radical new approach? BioEssays 32: 926-31.

223. Castellón, E., Chavarría, M., de Lorenzo, V., Zayat, M. and Levy, D. (2010) An electrooptical device from a biofilm structure created by bacterial activity. Advanced Materials 22: 4846-4850.

222. de las Heras, A., Carreño, C.A., Martínez E. and de Lorenzo, V. (2010) Engineering input/output nodes in prokaryotic regulatory circuits. FEMS Microbiology Reviews 34:842-65.

221. Koutinas, M., Lam, M. C., Kiparissides, A., Silva-Rocha, R., Godinho, M., Livingston, A. G., Pistikopoulos, E.N., de Lorenzo, V., Martins dos Santos VAP, Mantalaris, A. (2010). The regulatory logic of m-xylene biodegradation by Pseudomonas putida mt-2 exposed by dynamic modelling of the principal node Ps/Pr of the TOL plasmid. Env Micro 12: 1705-1718.

220. Tamames, J. and de Lorenzo, V. (2010) EnvMine: A text-mining system for the automatic extraction of contextual information. BMC Bioinformatics 11: 294.

219. Silva-Rocha, R and de Lorenzo, V. (2010) Noise and robusteness in prokaryotic regulatory Networks. Ann Rev Microbiol 64: 257-275

218. de Lorenzo, V. and Loza-Tavera, H. (2010) Microbial bioremediation of chemical pollutants: How bacteria cope with multi-stress environmental scenarios. In Bacterial Stress Responses (Gisela Storz and Regine Hengge, Eds). ASM Press, Washington DC, pp. 481-492

217. de Lorenzo, V., Silva-Rocha, R., Carbajosa, G. Galvão, T.C., and Cases, I. (2010) Sensing xenobiotic compounds: lessons from bacteria that face pollutants in the environment In Sensory Mechanisms in Bacteria (Ed. S. Spiro and R. Dixon). Horizon Scientific Press, Norwich, pp. 81-105.

216. Muñoz, A., Valls, M. and de Lorenzo, V. (2010) Extreme DNA bending: molecular basis of the regulatory breadth of IHF. In Bacterial Chromatin (Ed. Charles Dorman Springer-Verlag Berlin Heidelberg (Chapter 16) pp. 365-393.

215. de Lorenzo, V., Fraile, S. and Jiménez J.I. (2009) Emerging Systems and Synthetic Biology Approaches to Hydrocarbon Biotechnology. In Handbook of Hydrocarbon and Lipid Microbiology (Ed. K.N. Timmis) Springer-Verlag Berlin Heidelberg. Vol. 2. Microbiology of utilization of hydrocarbons, oils and lipids (Chapter 96).

214. de las Heras, A. and V. de Lorenzo (2009) Genetic constructs: molecular tools for the assembly of environmental bacterial biosensors. In Handbook of Hydrocarbon and Lipid Microbiology (Ed. K.N. Timmis) Springer-Verlag Berlin Heidelberg. Vol 3. Consequences of microbial interactions with hydrocarbons, oils and lipids (Chapter 19).

213. de Lorenzo, V. (2009) Exploiting Microbial diversity: the challenges and the means. In Handbook of Hydrocarbon and Lipid Microbiology (Ed. K.N. Timmis) Springer-Verlag Berlin Heidelberg. Vol 3. Consequences of Microbial interactions with hydrocarbons, oils and lipids. (Chapter 1).

212. Carreño, C.A and de Lorenzo, V. (2009) Genetic traps for surveying new catalysts in (meta)genomic DNA. In Handbook of Hydrocarbon and Lipid Microbiology (Ed. K.N. Timmis) Springer-Verlag Berlin Heidelberg. Vol. 4 Experimental Protocols and appendices (Chapter 96)

211. de Lorenzo, V. (2009). Recombinant bacteria for environmental release: what went wrong and what we have learnt from it. Clinical Microbiology and Infection 1: 63-65.

210. Páez-Espino, D., Tamames, J., de Lorenzo, V., Canovas, D. (2009) Microbial responses to environmental arsenic. BioMetals 22: 117-130

209. de Lorenzo V, Galperin M. (2009) Microbial systems biology: bottom up and top down. FEMS Microbiol Rev. 33: 1-2

208. de Lorenzo, V. (2008) Systems Biology approaches to Bioremediation. Curr Op Biotech 19: 579-89.

207. de Lorenzo, V. and Danchin, A. (2008) Synthetic biology: discovering new worlds and new words. The new and not so new aspects of this emerging research field. EMBO Rep 9: 822-827.

206. de las Heras, A., Carreño, CA, de Lorenzo, V. (2008) Stable implantation of orthogonal sensor circuits in Gram-negative bacteria for environmental release. Env Microbiol 10: 3305-3316.

205. Vitale, E., Milani, A., Renzi, F., Galli, E., Rescalli, E., de Lorenzo, V. and Bertoni, G. (2008) Transcriptional wiring of the TOL plasmid regulatory network to its host involves the submission of the 54-promoter Pu to the response regulator PprA. Mol Microbiol 69: 698-713

204. Pflüger, K., de Lorenzo, V. (2008) Evidence of in vivo cross-talk between the nitrogen-related and fructose-related branches of the carbohydrate phosphotransferase system of Pseudomonas putida. J. Bacteriol. 190: 3374-3380.

203. Silva-Rocha, R. and de Lorenzo (2008) Mining logic gates in prokaryotic transcriptional regulation networks. FEBS Lett 582: 1237-44.

202. Garmendia, J., de las Heras, A., Calcagno Galvão T. and de Lorenzo V. (2008) Tracing explosives in soil with transcriptional regulators of Pseudomonas putida evolved for responding to nitrotoluenes. Microb Biotech. 1: 236–246

201. Pflüger, K., di Bartolo, I. Velázquez, F. and de Lorenzo, V. (2007) Non-disruptive release of Pseudomonas putida proteins by in situ electric breakdown of intact cells. J. Microbiol. Meth. 71: 179-185.

200. Cases, I, Velázquez, F., and de Lorenzo, V. (2007) The ancestral role of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) exposed by comparative genomics. Res. Microbiol. 158: 666-670.

199. Galvão, T. C., Mencía M., de Lorenzo, V. (2007) Emergence of novel functions in transcriptional regulators by regression to stem protein types. Mol Microbiol. 65: 907–919.

198. Platero, R., de Lorenzo, V., Garat, B., Fabiano, E. (2007). The Sinorhizobium meliloti Fur like (Mur) protein binds a Fur box like sequence present in the mntA promoter in a manganese responsive manner. Appl. Env. Microbiol. 73: 4832-4838.

197. Pflüger, K., de Lorenzo, V. (2007) Growth-dependent phosphorylation of the PtsN (EIINtr) protein of Pseudomonas putida. J. Biol. Chem. 282: 18206-18211.

196. McFall-Ngai, M., de Lorenzo, V. (2007) The ultimate rendezvous: microbial ecology meets industrial biotechnology. Curr. Op. Microbiology. 10: 205-206.

195. Gómez, M.J., Pazos, F., Guijarro, F.J., de Lorenzo, V. and Valencia, A. (2007). The environmental fate of organic pollutants through the global microbial metabolism. Mol. Sys. Biol. 3: 114.

194. Cánovas, D. and de Lorenzo, V. (2007). Osmotic stress limits arsenic hyper-tolerance in Aspergillus sp. P37. FEMS Ecology 61: 258-263.

193. Velázquez, V. Pflüger, K., Cases, I., De Eugenio, L and de Lorenzo, V. (2007) The phosphotransferase system (PTS) formed by PtsP, PtsO, and PtsN proteins controls production of polyhydroxyalkanoates in Pseudomonas putida. J. Bacteriol. 189: 4529-4533.

192. Galvao, T., de Lorenzo, V. and Canovas D. (2006). Uncoupling of choline-O-sulfate utilisation from osmoprotection in Pseudomonas putida. Mol Microbiol 62, 1643-1654.

191. Mencia, M., Martínez-Ferri, A.I., Alcalde, M. and de Lorenzo, V. (2006) Identification of a -hexachlorocyclohexane dehydrochlorinase (LinA) variant with improved expression and solubility properties. Biocatal Biotransformation 24: 223-230.

190. de Lorenzo, V. (2006). Blueprint of an oil-eating bacterium. Nature Biotechnol. 24: 952-953.

189. Jurado., P., Fernandez, L.A. and de Lorenzo, V. (2006). In vivo drafting of single-chain antibodies for regulatory duty on the 54-promoter Pu of the TOL plasmid. Mol. Microbiol. 60: 1218-1227.

188. Velazquez, F., Fernandez, S. and de Lorenzo, V. (2006) The upstream activating sequences (UAS) of the 54 promoter Pu of Pseudomonas putida filter transcription readthrough from upstream genes. J Biol Chem 281:11940-11948.

187. Domínguez-Cuevas, P., González-Pastor, J.E., Marqués, S., Ramos, J.L. and de Lorenzo, V. (2006). Transcriptional tradeoff between metabolic and stress-response programs in Pseudomonas putida KT2440 cells exposed to toluene. J. Biol.. Chem 281: 11981-11991.

186. de Lorenzo, V., Serrano, L. and Valencia, A. (2006). Synthetic Biology: challenges ahead. Bioinformatics 22, 127-128

185. Jurado., P., de Lorenzo, V. and Fernández, L.A. (2006). Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of Escherichia coli: evidence that chaperone activity is the prime effect of thioredoxin. J. Mol. Biol. 357: 49-61.

184. Mohn, W., Garmendia, J., Galvao, T. and de Lorenzo, V. (2006). Surveying biotransformations with à la carte genetic traps: translating dehydrochlorination of lindane (-HCH) into lacZ-based phenotypes. Env. Microbiol. 8: 546-555.

183. Galvao, T. and de Lorenzo, V. (2006). Transcriptional regulators à la carte: engineering new effector specificities in bacterial regulatory proteins. Curr Op Biotech 17: 34-42.

182. Velázquez, F., de Lorenzo, V. and Valls, M. (2006). The m-xylene biodegradation capacity of Pseudomonas putida mt-2 is submitted to adaptation to abiotic stresses: evidence from expression profiling of xyl genes. Env. Microbiol. 8: 591–602

181. Yuste, L., Hervás, A.B., Canosa, I., Tobes, R., Jiménez, J.I., Nogales, J., Pérez-Pérez, M., Santero, S., Díaz, E., Ramos, J.L., de Lorenzo, V., and Rojo, F. (2006) Growth-phase dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analyzed with a genome-wide DNA microarray. Env. Microbiol. 8 : 165–177

180. Mohn, W., Mertens, B., Neufeld, J. D., Verstraete, W. de Lorenzo, V. (2006) Distribution and phylogeny of hexachlorocyclohexane-degrading bacteria in soils from Spain. Env. Microbiol. 8, 60-68.

179. Neufeld, J. D., Mohn, W., and de Lorenzo, V. (2006). Composition of microbial communities in hexachlorocyclohexane (HCH) contaminated soils from Spain revealed with a habitat-specific microarray. Env. Microbiol. 8, 126-140.

178. de Lorenzo, V. (2005) Problems with metagenomic screening. Nature Biotechnol. 23: 1045.

177. Cases I. and de Lorenzo, V. (2005). Genetically modified organisms for the environment: stories of success and failure and what we have learnt from them. Int. Microbiol. 8: 213-222

176. Galvao, T. and de Lorenzo, V. (2005). Surveying the biodegradation gene landscape by sequence-independent approaches. In F. Fava and P. Canepa (Eds). Innovatrive approaches to the Bioremediation of contaminated sites. Soil Remediation Series 6. INCA, Venice pp. 191-198.

175. Velázquez, F., Parro, V. and de Lorenzo, V. (2005). Inferring the genetic network of m-xylene metabolism through expression profiling of the xyl genes of Pseudomonas putida mt-2. Mol. Microbiol. 57: 1557-1569.

174. Juiz-Río, S., Osorio, C. R., de Lorenzo, V. and Lemos M. L. (2005) Substractive hybridization reveals a high genetic diversity in the fish pathogen Photobacterium damselae ssp. piscicida: evidence of a STX-like element. Microbiology 151: 2659-2669.

173. Galvao, T., Mohn, W. and de Lorenzo, V. (2005) Exploring the microbial biodegradation and biotransformation gene pool. Trends Biotech. 23: 497-506.

172. de Lorenzo, V. (2005) The second coming of physics into (micro)biology. Env. Microbiol. 7: 477-478

171. Guzmán, C. A., Cebolla, A., Beltrametti, F., Staender, L.H., de Lorenzo, V. (2005) Physiological stress of intracellular Shigella flexneri visualized with a metabolic sensor fused to a surface-reporter system. FEBS Lett. 579: 813-818.

170. Cases, I., and de Lorenzo, V. (2005) Promoters in the environment: transcriptional regulation in its natural context. Nature Microbiol. Revs. 3: 105-118.

169. Pazos F., D. Guijas, M. J. Gómez, A. Trigo, V. de Lorenzo y A. Valencia (2005). The biodegradation network, a new scenario for Computational Systems Biology Research. In Lecture Notes in Bioinformatics, Proceedings of the Second International Workshop on Computational Methods in Systems Biology. V. Danos and V. Schachter (Eds.). Springer-Verlag, Berlin-Heidelberg pp. 252- 256.

168. Galvao, T. and V. de Lorenzo (2005). Adapting the yeast URA3 selection system to Gram-negative bacteria: generation of a ∆betCDE Pseudomonas putida strain. Appl. Environ. Microbiol. 71: 883-992.
167. Pazos, F., Guijas, D., Valencia, A., de Lorenzo, V. (2005) MetaRouter: Bioinformatics for Bioremediation. Nucl. Acids Res. 33: D588-D592
166. Carmona, M., Fernandez, S., Rodriguez, M.J. and de Lorenzo, V. (2005) m-xylene responsive Pu/PnifH hybrid  promoters that overcome physiological control in Pseudomonas putida KT2442. J. Bacteriol. 187: 125-134.

165. Cánovas, D., Vooijs, R., Schat, H. and de Lorenzo, V. (2004) The role of thiol species in the hyper-tolerance of Aspergillus sp. P37 to arsenic. J. Biol. Chem. 279: 51234-51240.

164. Velázquez, F., di Bartolo, I. and de Lorenzo, V. (2004). Genetic evidence that catabolites of the Entner-Doudoroff pathway signal C-source repression of the sigma 54 Pu promoter of Pseudomonas putida . J. Bacteriol. 186: 8267-8275.

163. Mencía, V. and de Lorenzo, V. (2004) Functional implantation of the sumoylation machinery into Escherichia coli. Protein Expresión and Purification 37: 409-418.

162. Fraile, S., Muñoz, A., de Lorenzo, V. and Fernández, L.A. (2004). Secretion of proteins with dimerization capacity by the hemolysin type-I transport system of Escherichia coli. Mol. Microbiol. 53: 1109-1121.

161. Valls, M., Cases, I. and de Lorenzo, V. (2004) Transcription mediated by rpoN-dependent promoters. In J.L. Ramos (Ed). The Pseudomonads Vol II. Virulence and gene regulation. Kluver Academic / Plenum Publishers, New York. pp. 289-317.

160. Schweizer, H. and de Lorenzo, V. (2004) Molecular tools for genetic analysis of Pseudomonads. In J.L. Ramos (Ed). The Pseudomonads Vol I. Genomics, life style and molecular architecture. Kluver Academic / Plenum Publishers, New York. pp. 317-350.

159. Pugsley, A.P., Francetic, O., Driessen, A.J. and de Lorenzo, V. (2004) Getting out: protein traffic in prokaryotes. Mol. Microbiol. 52: 3-11.

158. Veiga, E., de Lorenzo, V. and Fernández, L.A. (2004). Structural tolerance of bacterial autotransporters for folded passenger protein domains. Mol. Microbiol. 52: 1069-1080.

157. Rescalli, E., Saini, S., Bartocci, C., Rychlewski, L., de Lorenzo, V. and Bertoni, G. (2004). Novel physiological modulation of the Pu promoter of TOL plasmid: negative regulatory role of the TurA protein of Pseudomonas putida in the response to sub-optimal growth temperatures. J. Biol. Chem. 279: 7777-7784.

156. Valls M., and de Lorenzo, V. (2003). Transient XylR binding to the UAS of the Pseudomonas putida 54 promoter Pu revealed with high intensity UV footprinting in vivo. Nucl. Acids. Res. 31: 6926-6934.

155. Veiga, E., de Lorenzo, V. and Fernandez, L.A. (2003). Neutralization of enteric coronaviruses with Escherichia coli cells expressing scFv-autotransporter fusions. J. Virology. 77: 13396-13398.

154. Cases, I. Ussery, D. and de Lorenzo, V. (2003) The 54 regulon (sigmulon) of Pseudomonas putida. Env. Microbiol. 5: 1281-1293.

153. Pazos, F., Valencia, A., and de Lorenzo, V. (2003) The organization of the microbial biodegradation network from a systems-Biology perspective. EMBO Reports 4: 994-999.

152. Canovas, D., Mukhopadhyay, R., Rosen, B. and V. de Lorenzo (2003). Arsenate transport and reduction in the hyper-tolerant fungus Aspergillus sp. P37. Env. Microbiol. 5: 1242-1256

151. Veiga, E., de Lorenzo, V. and Fernandez, L.A. (2003) Autotransporters as scaffolds for bacterial adhesins: surface properties of Escherichia coli cells displaying Jun/Fos dimerization domains. J. Bacteriol. 185: 5585-5590.

150. Macchi, R., Montesissa, L., Murakami, K., Ishihama, A., de Lorenzo, V. and Bertoni, G. (2003) Recruitment of 54-RNA polymerase to the Pu promoter of Pseudomonas putida through IHF-mediated positioning switch of CTD on an UP-like element. J. Biol. Chem. 278: 27695-27702.

149. van Dien, S. and de Lorenzo, V. (2003) Deciphering environmental signal integration in 54-dependent promoters with a simple mathematical model. J. Theor. Biol. 224: 437-449.

148. de Lorenzo, V., Pérez-Martín, J., Escolar, L., Pesole, G. and Bertoni, G. (2004). Mode of binding of the Fur protein to target DNA: negative regulation of iron-controlled gene expression. In Iron Transport in bacteria. Eds. J. Crosa, A.R. Mey and S. Payne. ASM Press, Washington. pp. 185-196.

147. Cases, I., de Lorenzo, V. and Ouzounis, C. (2003) Transcription regulation and environmental adaptation in bacteria. Trends in Microbiology 11: 248-53.

146. Jurado, P., Fernández, L.A. and de Lorenzo V. (2003). Sigma 54 levels and physiological control of the Pseudomonas putida Pu promoter. J. Bacteriol. 185: 3379-3383

145. Cánovas, C., Cases, I. and de Lorenzo, V. (2003) Heavy metal tolerance and metal homeostasis in Pseudomonas putida as revealed by complete genome analysis. Env. Microbiol. 5:1242-1256.

144. Kunin, V., Cases, I., Enright, A., de Lorenzo, V. and Ouzounis, C. (2003) Myriads of protein families, and still counting. Genome Biology 4: 401

143. Cánovas, D., C. Durán, N. Rodríguez, R. Amils and V. de Lorenzo (2003) Testing the limits of biological tolerance to arsenic in a fungus isolated from the River Tinto. Env. Microbiol. 5: 133-138.

142. Cebolla, A., Toyo, J.L., de Lorenzo, V. and Santero, E. (2002). Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression. Appl. Env. Microbiol. 68: 5034–5041.

141. Cases, I. and de Lorenzo, V. (2002). The grammar of (micro) biological diversity. Env. Microbiol. 4, 623-627

140. Valls, M. and de Lorenzo, V. (2002). Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution. FEMS Microbiol. Revs. 26: 327-338.

139. Jurado, P., Ritz, D., Beckwith, J., de Lorenzo, V., and Fernández, L.A. (2002). Production of functional single chain Fv antibodies in the cytoplasm of Escherichia coli. J. Mol. Biol. 320: 1–10

138. Veiga, E., Sugawara, E., Nikaido, H., de Lorenzo, V. and Fernández, L. A. (2002) Export of autotransported proteins proceeds through a oligomeric ring shaped by C-terminal domains. EMBO J. 21: 2122-2131.

137. Cases, I. and de Lorenzo, V. (2002). The genomes of Pseudomonas encode a third HU protein. Microbiology 148: 1243-1245

136. de Lorenzo (2002). Towards the end of experimental (micro)biology?. Environ. Microbiol. 4: 6-9.

135. Devos, D., Garmendia, J., de Lorenzo, V. and Valencia, A. (2002). Deciphering the action of aromatic affectors on the prokaryotic enhancer binding protein XylR through a threading model approach. Env. Microbiol. 4: 29-41.

134. Valls, M., Buckle, . and de Lorenzo, V. (2002). In vivo UV-laser footprinting of the Pseudomonas putida 54 Pu promoter reveals that IHF couples transcriptional activity to growth phase. J. Biol. Chem. 277: 2169–2175.

133. Fraile, S., Roncal, F., Fernández, L.A., and de Lorenzo, V. (2001) Monitoring intracellular levels of XylR in Pseudomonas putida with a single-chain antibody specific for aromatic-responsive enhancer-binding proteins. J. Bacteriol. 183: 5571-5579

132. Garmendia, J., Devos, D., Valencia, A. and de Lorenzo, V. (2001) À la carte transcriptional regulators: unlocking responses of the prokaryotic enhancer binding protein XylR to non-natural effectors. Mol. Microbiol. 42: 47-59

131. Cases, I., Velazquez, F. and de Lorenzo, V. (2001). The role of ptsO in carbon-mediated inhibition of the Pu promoter belonging to the pWW0 Pseudomonas putida plasmid. J. Bacteriol. 183: 5128-5133.

130. Busby S. and de Lorenzo V. (2001) Cell regulation. Putting together pieces of the big puzzle. Curr Opin Microbiol. 4: 117-118.

129. de Lorenzo, V. (2001) Cleaning up behind us. The potential of genetically modified bacteria to break down toxic pollutants in the environment. EMBO Reports 2: 357-359.

128. Arenghi, F.L, Barbieri, P., Bertoni, G. and de Lorenzo, V. (2001) New insights into the activation of o-xylene biodegradation Pseudomonas stutzeri OX1 by pathway substrates. EMBO Reports 2: 409-414.

127. Fernández, L.A. and de Lorenzo, V. (2001) Formation of disulfide bonds during secretion of proteins through the periplasmic-independent type I pathway. Mol. Microbiol. 40: 332-346.

126. Bartels, F., S. Fernández, A. Holtel, K. Timmis and V. de Lorenzo (2001). The essential HupB and HupN proteins of Pseudomonas putida provide redundant and non specific DNA bending functions. J. Biol. Chem. 276: 16641-16648

125. Cases, I and de Lorenzo, V. (2001) The limits to genomic predictions: role of N in environmental stress survival of Pseudomonas putida. FEMS Ecology 35: 217-221.

124. Cebolla, A, Sousa, C, and de Lorenzo, V. (2001). Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucl. Acids Res. 29: 759-766

123. Cases, I., López, J. A., Albar, J. P. and de Lorenzo, V. (2001) Evidence of multiple regulatory functions for the PtsN (IIANtr) protein of Pseudomonas putida. J. Bacteriol. 183: 1032-1037.

122. Cases, I and de Lorenzo, V. (2001) The black cat/white cat principle of signal integration in bacterial promoters. EMBO J. 20: 1-11.

121. Haro, A. and V. de Lorenzo (2001) Metabolic engineering of bacteria for environmental applications: construction Pseudomonas strains for biodegradation of 2-chlorotoluene. J. Biotechnol. 85: 103-113

120. de Lorenzo, V. and Fernández, J. (2000). Expression vectors and delivery systems: playing alien genes in remote theaters. Curr. Op. Biotechnol. 11: 427-428.

119. Fernández, L.A., I. Solá, L. Enjuanes and V. de Lorenzo (2000) Specific secretion of active single-chain Fv antibodies to the supernatants of Escherichia coli cultures using the hemolysin system. Appl. Env. Microbiol. 66, 5024-5029.

118. Garmendia, J. and V. de Lorenzo (2000). The role of the inter-domain B linker in the activation of the XylR protein of Pseudomonas putida. Mol. Microbiol. 38, 401-410.

117. Carmona, M., M.J. Rodríguez, O. Martínez-Costa and V. de Lorenzo (2000) In vivo and in vitro effects of (p)ppGpp on the 54 promoter Pu of the TOL plasmid of Pseudomonas putida. J. Bacteriol. 182: 4711-4718.

116. Escolar, L., J. Pérez-Martín and V. de Lorenzo (2000) Evidence of an unusually long operator for the Fur (ferric uptake) repressor in the aerobactin promoter of Escherichia coli. J. Biol. Chem. 275: 24709-24714.

115. Garmendia, J. and de Lorenzo, V. (2000) Visualization of DNA-protein intermediates during activation of the Pu promoter of the TOL plasmid of Pseudomonas putida. Microbiology 146, 2555-2563.

114. de Lorenzo, V. (2000) Pseudomonas entering the post-genomic era Env. Microbiol. 2: 349-354.

113. Valls, M., S. Atrian, V. de Lorenzo and L. A. Fernández (2000) Engineering a mouse metallothionein on the cell surface of Ralstonia eutropha CH34 for enhanced immobilization of heavy metals in soil. Nature Biotechnology 18: 661-665.

112. de Lorenzo, V. (2000). La investigación Biomédica. Arbor 653: 17-36

111. Leoni, L., Orsi, N. de Lorenzo, V., and Visca, P. (2000). Functional analysis of PvdS, an iron starvation sigma factor of Pseudomonas aeruginosa. J. Bacteriol. 182: 1481-1491.

110. Cases, I and de Lorenzo, V. (2000) Genetic evidence of distinct physiological regulation mechanisms in the 54 Pu promoter of Pseudomonas putida. J. Bacteriol. 182: 956-960.

109. Kaldalu N., U. Toots, V. de Lorenzo, and M. Ustav (2000). Functional domains of the TOL plasmid transcription factor. J. Bacteriol. 182: 1118-1126.

108. de Lorenzo, V. (1999). Organismos modificados genéticamente y bioremediación. En Promoción y difusión de la Biotecnología en España: un dialogo prospectivo con la Administración. Ed. Muñoz, E. y Bas F. CEFI-GABIOTEC, Madrid. pp. 121-128

107. Panke, S., V. de Lorenzo, A. Kaiser, B. Witholt, and M. Wubbolts (1999) Engineering of a Stable Whole-Cell Biocatalyst Capable of (S)-Styrene Oxide Formation for Continuous Two-Liquid-Phase Applications. Appl. Envir. Microbiol. 65: 5619-5623.

106. Carmona, M., de Lorenzo, V. Bertoni, G. (1999). Recruitment of RNA polymerase is a rate-limiting step for the activation of the 54 promoter Pu of Pseudomonas putida. J. Biol. Chem. 274: 33790-33794.

105. Valls, M., de Lorenzo, V., González-Duarte, R., Atrian, S. (2000). Engineering of Pseudomonas outer membrane proteins for enhanced heavy-metal bioadsorption. J. Inorganic Biochemistry 79: 219-223

104. Escolar, L., Pérez-Martín, J. and de Lorenzo, V. (1999) Opening the iron box: transcriptional metalloregulation by the Fur protein. J. Bacteriol. 181: 6223-6229.

103. Veiga, E., Fernández, L.A. and de Lorenzo, V. (1999). Probing secretion and translocation of a beta-autotransporter using a reporter single-chain Fv as a cognate passenger domain. Mol. Microbiol. 33: 1232-1243.

102. de Lorenzo, V. and Kuenen, G. (1999). Scientific basis for the remediation of the toxic spill of the Aznalcóllar mine : combining bacteria and plants to address an intractable kind of pollution. Enviromental Microbiology 1: 275-278.

101. Vicente, M., Chater, K. and de Lorenzo, V. (1999) Bacterial Transcription Factors involved in Global Regulation. Mol. Microbiol. 33: 8-17

100. Cases, I., Pérez-Martín, J. and de Lorenzo, V. (1999) The IIANtr (PtsN) protein of Pseudomonas putida mediates the C-source inhibition of the 54-dependent Pu promoter of the TOL plasmid. J. Biol. Chem. 274: 15562-15568.

99. Kotrba, P., Dolecková, L., de Lorenzo, V. and Ruml, T. (1999). Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl. Environ. Microbiol. 65: 1092-1098.

98. Carmona, M. and de Lorenzo, V. (1999) Involvement of the FtsH (HflB) protease in the activity of 54 promoters. Mol. Microbiol. 31: 261-270.

97. Corbisier, P., van der Lelie, D., Borremans, B., Provost, A., de Lorenzo, V., Brown, N., Lloyd, J.R., Hobman J.L., Csöregi, E., Johansson, G., and Mattiasson, B. (1999) Whole cell and protein-based biosensors for the detection of bioavailable heavy metals in environmental samples. Anal. Chim. Acta 387: 235-244.

96. Lau, P., and de Lorenzo, V. (1999) Genetic engineering: the frontier of bioremediation. Environmental Science & Technology (News & Reseach Notes) 4: 124A-128A.

95. Sánchez-Romero, J. M. and V. de Lorenzo. 1999. Genetic engineering of non-pathogenic Pseudomonas strains as biocatalysts for industrial and environmental processes. In Manual of Industrial Biotechnology (2nd ed). J. Davies, G. Cohen (ed). American Association of Microbiology Press, Washington DC. pp. 460-474.

94. Kotrba P., Pospisil P., de Lorenzo V., Ruml T. (1999). Enhanced metallosorption of Escherichia coli cells due to surface display of beta- and alpha-domains of mammalian metallothioneins as a fusion to LamB Protein. Journal of Receptor Research and Signal Transduction 19: 703-715.

93. Diaz, E., Chitila-Munthali, M.T., Jaenecke, S., de Lorenzo V. and Timmis, K.N. (1999). Design of genetic circuits for restricting gene and biocatalyst dispersal. In Molecular Microbial Ecology Manual. 6.1.14. Eds. A.D.L. Akkermans, J.D. van Elsas, and F.J. de Bruijn. Kluwer Academic Publishers. pp. 1-18.

92. Valls, M., González-Duarte, R., Atrian, S, de Lorenzo, V. (1998). Bioaccumulation of heavy metals with protein fusions of metallothionein to bacterial OMPs. Biochimie 80: 855-861.

91. Escolar, L., Pérez-Martín, J. and de Lorenzo, V. (1998) Binding of the Fur (ferric uptake regulator) repressor of Escherichia coli to arrays of the GATAAT sequence. J. Mol. Biol. 283: 537-547

90. Sánchez-Romero, J.M., Díaz R. and de Lorenzo, V. (1998) Resistance to tellurite as a selection marker for genetic manipulations of Pseudomonas strains. Appl. Env. Microbiol. 64 : 4040-4046

89. Bertoni, G., Fujita, N., Ishihama, A. and de Lorenzo, V. (1998) Active recruitment of 54-RNA polymerase to the Pu promoter of Pseudomonas putida role of IHF and CTD. EMBO J. 17 : 5120-5128.

88. de Lorenzo, V., Herrero, M., Sánchez, J.M. and Timmis, K. N. 1998 Mini-transposons in microbial ecology and environmental biotechnology. FEMS Microbiol. Ecology 27: 211-224.

87. Escolar, L., J. Pérez-Martín and V. de Lorenzo. 1998. Coordinated repression in vitro of the divergent fepA-fes promoters of Escherichia coli by the iron uptake regulation (Fur) protein. J. Bacteriol. 180: 2579-2582.

86. Sousa, C., P. Kotrba, T. Ruml, A. Cebolla and V. de Lorenzo. 1998. Enhanced metalloadsorption of Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB. J. Bacteriol. 180: 2280-2284.

85. Cases, I. and de Lorenzo V. 1998. Expression systems and physiological control of promoter activity in bacteria. Curr. Op. Microbiol. 1 : 303-306.

84. Panke, S., Sánchez-Romero, J. M. and de Lorenzo, V. 1998. Engineering quasi-natural Pseudomonas putida strains for metabolism of toluene through an ortho-cleavage degradation pathway. Appl. Environ. Microbiol. 64 : 748-751.

83. Bertoni, G. Marqués, S. and de Lorenzo, V. 1998. Activation of the toluene-responsive regulator XylR causes a transcriptional switch between s54 and s70 promoters at the divergent Pr/Ps region of the TOL plasmid. Mol. Microbiol. 27 : 651-659.

82. Ramos, J.L. and de Lorenzo, V. 1997. Marcadores inocuos para el seguimiento de microbios en el medio ambiente. En El Libro Verde de la Biotecnología en la Agricultura (Ed. A. Albert). SEBIOT Madrid, pp. 75-80.

81. de Lorenzo, V. and Timmis, K. 1997. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5 and Tn10-derived mini-transposons. In Bacterial Pathogenesis (V.L. Clark and P.M. Bavoil, eds.) Selected Methods in Enzymology. Academic Press, San Diego, California. p. 227-246.

80. Gallardo, M. E., Fernández, A., de Lorenzo, V., García, J.L. and Díaz, E. 1997. Designing recombinant Pseudomonas strains to enhance biodesulfurization. J. Bacteriology 179: 7156-7160.

79. Escolar, L., V. de Lorenzo and J. Pérez-Martín. 1997. Metalloregulation in vitro of the aerobactin promoter of Escherichia coli by the Fur (ferric uptake regulation) protein. Mol. Microbiol. 26 : 799-808.

78. Pérez-Martín, J., I. Cases and V. de Lorenzo. 1997. Design of a solubilization pathway for recombinant polypeptides in vivo through processing of a bi-protein with a viral protease. Prot. Eng. 10 : 725-730.

77. Sousa, C., V. de Lorenzo and A. Cebolla. 1997. Modulation of gene expression through chromosomal positioning. Microbiology 143: 2071-2078.

76. Pérez-Martín, J. and de Lorenzo, V. 1997. Clues and consequences of DNA bending in transcription. Ann. Rev. Microbiol. 51 : 593-628.

75. de Lorenzo, V. (1997) Bioremediation : more than cloning and pasting DNA. Trends in Biotechnology 15 : 235-236.

74. Pérez-Martín, J. and V. de Lorenzo. 1997. Co-activation in vitro of the 54 dependent promoter Pu of the TOL plasmid of Pseudomonas putida by the prokaryotic histone HU and the mammalian HMG-1 protein. J. Bacteriol. 179: 2757-2760.

73. Bertoni, G., Pérez-Martín, J. and V. de Lorenzo. 1997. Genetic evidence of separate repressor and activator activities of the XylR regulator of the TOL plasmid pWW0 of Pseudomonas putida. Mol. Microbiol. 23 : 1221-1227.

72. Cebolla, A., C. Sousa and V. de Lorenzo (1997) Effector-specificity mutants of the transcriptional activator NahR of naphthalene-degrading Pseudomonas define protein sites involved in binding of aromatic inducers. J. Biol. Chem. 272 : 3986-3992.

71. de Lorenzo, V. 1997. De espada a arado: Hemolisina de Escherichia coli. Investigación y Ciencia 245 : 38-39

70. Calb, R., A. Davidovitch, S. Koby, H. Giladi, D. Goldenberg, H. Margalit, A. Holtel, K. Timmis, J. M. Sanchez-Romero, V. de Lorenzo and A. B. Oppenheim. 1996. Structure and function of the Pseudomonas putida integration host factor. J. Bacteriology 178 : 6319-6326

69. Tzchaschel, B., Klee, S., de Lorenzo, V., Timmis, K.N., and Guzmán, C. 1996. Towards a vaccine candidate against Shigella dysenteriae 1 : expression of the Shiga toxin B subunit in an attenuated Shigella flexneri aroD carrier strain. Microbial Pathogenesis 21, 277-288.

68. Sousa, C., Cebolla, A. and de Lorenzo, V. 1996. Enhanced metallo-adsorption and metallotropism of bacterial cells displaying poly-His peptides on their outer membrane. Nature Biotechnology 14, 1017-1020.

67. Pérez-Martín, J. and de Lorenzo, V. 1996. ATP binding to the s54-dependent activator XylR triggers a protein multimerization cycle catalysed by UAS DNA. Cell 86, 331-339

66. Jaenecke, S., de Lorenzo, V., Timmis, K. N. and Díaz, E. 1996. A stringently-controlled expression system to analyse lateral gene transfer between bacteria. Molecular Microbiology 21: 293-300

65. Pérez-Martín, J. and de Lorenzo, V. 1996. VTR expression cassettes for engineering conditional phenotypes in Pseudomonas : Activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein. Gene 172 : 81-86.

64. Tzchaschel, B., Guzmán, C., Timmis, K. N., and de Lorenzo, V. 1996. An Escherichia coli hemolysin transport system-based vector for the export of polypeptides : Export of Shiga-like Toxin IIe B subunit by Salmonella typhimurium aroA. Nature Biotechnology 14: 765-769.

63. Fernández, S., Pérez-Martín, J. and de Lorenzo, V. (1996) Specifity and promiscuity of catabolic promoters of Pseudomonas. In T. Nakazawa (ed.) Pseudomonads : Biotechnology and Molecular Biology. American Association of Microbiology. Washington D. C. pp. 165-175.

62. Pérez-Martín, J. and de Lorenzo, V. 1996. Identification of the repressor subdomain within the signal reception module of the prokaryotic enhancer-binding protein XylR of Pseudomonas putida. J. Biol. Chem. 271 : 7899-7902

61. Pérez-Martín, J. and de Lorenzo, V. 1996. Physical and functional analysis of the prokaryotic enhancer of the s54-promoters of the TOL plasmid of Pseudomonas putida. J. Mol. Biol. 258 : 562-574.

60. Pérez-Martín, J. and de Lorenzo, V. 1996. In vitro activities of an N-terminal truncated form of XylR, a 54-dependent transcriptional activator of Pseudomonas putida. J. Mol. Biol. 258 : 575-587.

59. Lechuga, L. y de Lorenzo, V. 1996. El uso de biosensores en medicina y medio ambiente. Fronteras de la Ciencia y la Tecnología 12 : 18-21.

58. de Lorenzo, V. and Pérez-Martín, J. 1996. Regulatory noise in prokaryotic promoters : How bacteria learn to respond to novel environmental signals. Mol. Microbiol. 19 : 1177-1184.

57. Cebolla, A., Guzmán, C. and de Lorenzo. V. 1996. Non-disruptive detection of activity of catabolic promoters of Pseudomonas with an antigenic surface reporter system. Applied and Environmental Microbiology 62 : 214-220.

56. Cases, I., V. de Lorenzo and J. Pérez-Martín. 1996. Involvement of sigma factor s54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter. Mol. Microbiol. 19 : 7-17.

55. Pérez-Martín, J. and de Lorenzo, V. 1995. Las estructuras del ADN como elementos auxiliares en la regulación de la transcripción. En Microbiología y Genética Molecular : Ed. : J. Casadesús. pp. 173-189. Sociedad Española de Microbiología y Servicio de Publicaciones de la Universidad de Huelva.

54. Pérez-Martín, J. and V. de Lorenzo. 1995. The N-terminal domain of the prokaryotic enhancer-binding protein XylR is a specific intramolecular repressor. Proc. Natl. Acad. Sci. USA 92 : 9392-9396.

53. Pérez-Martín, J. and V. de Lorenzo. 1995. The 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR. Journal of Bacteriology 177 : 3758-3763.

52. Pérez-Martín, J. and V. de Lorenzo. 1995. Integration Host Factor (IHF) supresses promiscuous activation of the 54-dependent promoter Pu of Pseudomonas putida. Proc. Natl. Acad. Sci. USA 92 : 7277-7281.

51. de Lorenzo, V. 1995. Biotecnología medioambiental : Nuevos abordajes biológicos al problema de los residuos industriales. En La Biotecnología y su aplicación industrial en España. Eds : J. L. Carrascosa y A. Modrego. pp. 123-130. Consejo Superior de Investigaciones Científicas. Madrid.

50. Fernández, S., V. de Lorenzo and J. Pérez-Martín. 1995. Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains. Molecular Microbiology 16 : 205-213

49. Brazil. D., L. Kenefick, M. Callanan, A. Haro, V. de Lorenzo, D. N. Dowling and F. O’Gara. 1995. Construction of a rhizosphere Pseudomonad with potential to degrade polychorinated biphenyls and detection of bph gene expression in the rhizosphere. Applied and Enviromental Microbiology 61: 1946-1952

48. Kristensen, C., Eberl, L., J. M.Sánchez-Romero, J. M. Giskov, M., S. Molin and V. de Lorenzo. 1995. Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4. J. Bacteriol. 177 : 52-58

47. Martínez, J. L. and V. de Lorenzo (1995) Molecular strategies of iron adquisition by bacteria : The Fur protein and the aerobactin case history (J. Abadía, Ed) Iron mutrition in soils and plants. Kluwer Academic Publishers, The Netherlands. pp. 335-341.

46. Ramos, J. L., E. Díaz, D. Dowling, V. de Lorenzo, S. Molin, F. O´Gara, C. Ramos, K. N. Timmis. 1994. Behavior of bacteria designed for biodegradation. Bio/Technology 12 : 1349-1356.

45. Kessler B., K. N. Timmis, V. de Lorenzo. 1994. The organization of the Pm promoter of the TOL plasmid reflects the structure of its cognate activator protein XylS. Molecular and General Genetics 244 : 596-605

44. Pérez-Martín, J., K. N. Timmis and V. de Lorenzo. 1994. Co-regulation by bent DNA : Functional substitutions of the IHF site at the s54-dependent promoter Pu of the upper-TOL operon by intrinsically curved sequences.Journal of Biological Chemistry 269 : 22657-22662

43. Díaz, E., M. Munthali, V. de Lorenzo and K. N. Timmis. 1994. Universal barrier to lateral spread of specific genes among microorganisms. Molecular Microbiology 13 : 855-861

42. Kessler, B., S. Marqués, T. Köhler, J. L. Ramos, K. N. Timmis, and V. de Lorenzo. 1994. Cross-talk between catabolic pathways in Pseudomonas : XylS-dependent and independent activation of the TOL meta-operon require the same cis-acting sequences within the Pm promoter. Journal of Bacteriology 176: 5578-5582.

41. Fernández, S., V. Shingler and V. de Lorenzo. 1994. Cross-regulation by XylR and DmpR activators of Pseudomonas putida suggests that transcriptional control of biodegradative operons evolves independently of catabolic genes Journal of Bacteriology 176 : 5052-5058

40. Pérez-Martín, J., F. Rojo and V. de Lorenzo. 1994. Promoters responsive to DNA bending : A common theme in prokaryotic gene expression. Microbiological Reviews 58 : 268-290

39. V. de Lorenzo (1994) Genetic strategies to engineer expression systems responsive to relevant environmental signals (F. O´Gara, D. Dowling, B. Boesten, Eds.). pp. 91-101. Molecular Ecology of Rhizosphere Microorganisms. VCH, Weinheim

38. de Lorenzo, V. (1994) Designing microbial systems for gene expression in the field. Trends in Biotechnology 12 : 365-371

37. Kessler, B., M. Herrero, K. N. Timmis and V. de Lorenzo. 1994. Genetic evidence that the XylS regulator of the TOL meta-operon of Pseudomonas controls Pm promoter through weak DNA-protein interactions. Journal of Bacteriology 176 : 3171-3176

36. Martínez, J. L., Herrero, M. and de Lorenzo, V. 1994. The organization of intercistronic regions of the aerobactin operon of pColV-K30 may account for the differential expression of the iucABCD iutA genes. Journal of Molecular Biology 238 : 288-293

35. O´Sullivan, D. J., D. N. Dowling, V. de Lorenzo and F. O´Gara. 1994. Escherichia coli ferric uptake regulator (Fur) can mediate regulation of a pseudomonad iron-regulated promoter. FEMS Microbiology Letters 117 : 327-332

34. de Lorenzo, V. (1994) “Touching an elephant in the darkness” or How to get the whole picture of Biotechnology. Trends in Biotechnology 12 : 2-5

33. de Lorenzo, V. and Timmis, K. (1994) Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5 and Tn10-derived mini-transposons. Methods in Enzymology 235 : 386-405

32. de Lorenzo, V. and F. Rojo. 1994. Ingeniería genética de bacterias para aplicaciones medioambientales. In M. Vicente (ed.) Avances en Ingeniería Genética. Consejo Superior de Investigaciones Científicas. Madrid. pp. 333-358

31. de Lorenzo,V., Cases, I., Herrero, M. and Timmis, K. N. 1993. Early and late responses of TOL promoters to pathway inducers : Identification of Growth-phase dependent promoters in Pseudomonas putida with lacZ-tet bicistronic reporters. Journal of Bacteriology 175 : 6902-6907

30. Herrero, M., de Lorenzo., V., Ensley, B. and Timmis, K. N. 1993. A T7 RNA polymerase-based system for the construction of Pseudomonas strains with phenotypes dependent on TOL-meta pathway effectors. Gene 134 : 103-106

29. Kessler,B., de Lorenzo,V., Timmis, K. (1993) Identification of a cis-acting sequence within the Pm promoter of the TOL plasmid which confers XylS-mediated responsiveness to substituted benzoates. Journal of Molecular Biology 230 : 699-703

28. de Lorenzo,V., Fernández, S., Herrero, M. and Timmis, K. (1993) Engineering of alkyl- and haloaromatic-responsive gene expression with mini-transposons containing regulated promoters of biodegradative pathways of Pseudomonas. Gene 130 : 41-46

27. de Lorenzo, V., Eltis, L., Kessler, B. and Timmis, K. (1993). Analysis of gene products of Pseudomonas with lacIq/Ptrp-lac plasmids and transposons conferring conditional phenotypes. Gene 123 : 17-24

26. Su, G., Brahmbhatt, H., de Lorenzo, V., Wehland, J. and Timmis, K. N. 1992. Extracellular export of Shiga toxin B-subunit/haemolysin A (C-terminus) fusion protein expressed in Salmonella typhimurium aroA-mutant and stimulation of ß-subunit specific antibody responses in mice. Microbial Pathogenesis 13 : 465-476

25. Kessler, B., de Lorenzo,V. and Timmis,K.N. 1992. A general system to integrate lacZ fusions into the chromosomes of Gram-negative eubacteria : Regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy. Molecular and General Genetics 233 : 293-301

24. Michán, C., Kessler, B., de Lorenzo, V., Timmis, K. and Ramos, J. L. 1992. XylS domain interactions can be deduced from intraallelic dominance in double mutants. Molecular and General Genetics 235 : 406-412

23. de Lorenzo, V. and Timmis, K. N. (1992). Specialized host-vector systems for the engineering of Pseudomonas strains destined for environmental release. In Pseudomonads : Molecular Biology and Biotechnology. American Association of Microbiology. Washington D.C. pp. 415-428

22. de Lorenzo, V. 1992. Genetic engineering strategies for environmental applications. Current Opinion in Biotechnology 3 : 227-231

21. de Lorenzo, V., Herrero, M., Metzke, M. and Timmis, K. N. (1991). An upstream XylR- and IHF-induced nucleoprotein complex regulates the s54-dependent Pu promoter of TOL plasmid. EMBO Journal 10 : 1159-1167

20. Buyer, J., de Lorenzo, V. and Neilands, J. B. 1991. Production of siderophore aerobactin by a halophilic Pseudomonad. Applied and Environmental Microbiology 57: 2246-2250

19. Herrero, M., de Lorenzo, V. and Timmis, K. N. (1990). Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. Journal of Bacteriology 172 : 6557-6567

18. de Lorenzo, Herrero, M. and Timmis, K. N. (1990). Mini-Tn5 transposon derivatives for Insertion mutagenesis, promoter probing and chromosomal insertion of Cloned DNA in Gram-negative bacteria. Journal of Bacteriology 172 : 6568-6572

17. Nakamura,K., de Lorenzo,V, and Neilands, J. B. (1989). The Fur regulon of Escherichia coli K12. In Metal-DNA chemistry p. 106-108. (Ed. T.D. Tullius). American Chemical Society Symposium Series 402. Washington D.C.

16. Herrero, M., de Lorenzo,V. and Neilands,J.B. (1988) Nucleotide sequence of the iucD gene of the aerobactin operon of pColV-K30 and topology of its product studied with phoA and lacZ gene fusions. Journal of Bacteriology 170 : 56-64.

15. de Lorenzo,V., Herrero,M. and Neilands, J.B. (1988). pCON4 and pCON5 : Improved plasmid vectors to study bacterial promoters. FEMS Microbiology Letters 50 : 17-23.

14. de Lorenzo,V., Herrero,M., Giovannini,F., and Neilands, J.B. (1988). Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli. European Journal of Biochemistry 173 : 537-546.

13. de Lorenzo,V., Herrero,M. and Neilands, J.B. (1988). IS1-mediated mobility of the aerobactin system of pColV-K30 in Escherichia coli . Molecular and General Genetics 213 : 487-490.

12. de Lorenzo,V., Giovannini,F., Herrero,M. and Neilands, J. B. (1988). Metal ion regulation of gene expression : Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30. Journal of Molecular Biology 203 : 875-884.

11. de Lorenzo,V. and Martínez,J.L. (1988). Aerobactin production as a virulence factor: a re-evaluation. European Journal of Clinical Microbiology 7 : 621-629.

10. de Lorenzo,V., Wee,S., Herrero,M. and Neilands, J.B. 1987. Operator sequences of the Aerobactin Operon of plasmid ColV-K30 binding the ferric uptake regulation fur repressor. Journal of Bacteriology 169 : 2624-2630.

9. de Lorenzo, V., Bindereif, A., Paw, B. and Neilands, J. B. 1986. Aerobactin biosynthesis and transport genes of plasmid pColV-K30 in Escherichia coli K12. Journal of Bacteriology 165 : 570-578.

8. de Lorenzo, V. and Neilands, J. B. 1986. Characterization of iucA and iucC genes of the Aerobactin system of pColV-K30. Journal of Bacteriology 167 : 350-355.

7. Pugsley, A. P., Moreno, F. and de Lorenzo,V. 1986. Microcin E492-insensitive mutants of Escherichia coli K12. Journal of General Microbiology 132 : 3253-3259.

6. de Lorenzo,V. and Pugsley,A.P. (1985). Microcin E492, a low molecular weight antibiotic which causes depolarization of the Escherichia coli cytoplasmic membrane. Antimicrobial Agents and Chemotherapy 27 : 666-669.

5. de Lorenzo, V. l985. Factors affecting microcin E492 production. Journal of Antibiotics 38 : 340-343.

4. de Lorenzo,V., Martínez, J. L. and Asensio, C. 1984. Microcin mediated interactions between Klebsiella pneumoniae and Escherichia coli strains. Journal of General Microbiology 130 : 391-400.

3. de Lorenzo,V. and Aguilar, A. 1984. Antibiotics from Gram-negative bacteria: Do they play a role in microbial ecology? Trends in Biochemical Sciences 9 : 266-269.

2. de Lorenzo, V. 1984. Isolation and characterization of microcin E492 from Klebsiella pneumoniae . Archives of Microbiology 139 : 72-75.

1. de Lorenzo, V., Aguilar, A. and Asensio, C. 1982. Effect of D-serine on the antibiotic inhibition of one-carbon metabolism. FEMS Microbiology Letters 13 : 17-20 .