5Oct
Wednesday
08:30 - 09:30 Registration + Poster setup
09:30 - 09:35 Opening
09:35 - 10:55 Session I: Super-resolution. Invited talks

Chair: Timo Zimmermann. CRG-Centre for Genomic Regulations. Barcelona, Spain.

Democratising live-cell high-speed low-illumination super-resolution microscopy through Super-Resolution Radial Fluctuations. Ricardo Henriques.

Structured Illumination and the Analysis of Single Molecules in Cells. Rainer Heintzmann.

10:55 - 11:25 Poster flash (P1-P12)
11:25 - 12:00 Posters + Coffee
12:00 - 12:30 Session I: Super-resolution. Contributed talks

Chair: Jan Tonnesen. Institut Interdisciplinaire de Neurosciences. Bordeaux, France.

STED In-vivo imaging in the far red. Timo Zimmermann.

Platinum Shadowing for correlative light and electron microscopy. José Mª Mateos.

12:30 - 13:00 Poster flash (P13-P24)
13:00 - 14:00 On-site equipment workshops (parallel x3)

Nikon superior confocal performance: Enhanced resolution and spectral flexibility.

Zeiss Airyscan goes Fast: an innovative confocal imaging technology allows high resolution, high speed and low noise - simultaneously.

Leica TCS-SP8x: flexible excitation and super resolution.

14:00 - 15:00 Buffet lunch
15:00 - 15:55 Session II: Advanced Light Microscopy in neurosciences. Invited and Contributed talks

Chair: Asier Ruiz. Achucarro Basque Center for Neuroscience.

Applying STED microscopy to understand the electrical properties of dendritic spines. Jan Tønnesen. (Invited speaker).

Automatic analysis of microglial motility with ImageJ. Jorge Valero.

15:55 - 16:55 On-site equipment workshops (parallel x3)

Zeiss Airyscan goes Fast: an innovative confocal imaging technology allows high resolution, high speed and low noise - simultaneously.

Leica TCS-SP8x: flexible excitation and super resolution.

Advances in instrumentation and labels for highly multiplexed biological imaging.

16:55 - 17:30 Coffee break
17:30 - 18:30 Keynote Lecture

Super-resolution imaging of brain extracellular space in live tissue. Valentin Nägerl.

18:30 - 20:00 Posters + Welcome Reception
6Oct
Thursday
08:30 - 9:00 Registration
09:00 - 11:05 Session III: Quantitative Microscopy & Biophysics. Invited and contributed talks

Chair: Jose Requejo. Instituto Biofisika. (CSIC, UPV/EHU).

Statistical analysis of super-resolution microscopy data. Dylan Owen. (Invited speaker).

How biomimetic nanotemplates help resolving molecular details of cellular membrane fission. Vadim Frolov. (Invited speaker).

Elucidating Bax assembly and pore formation in membranes by advanced microscopy methods. Katia Consentino. (Invited speaker).

Analysis of nanoscale compartmentalization of PI(4,5)P2 in the plasma membrane through FRET imaging. Fábio Fernandes.

11:05 - 11:35 Posters + Coffee
11:35 - 13:00 Session IV: Functional imaging. Invited and contributed talks

Chair: Ricardo Andrade. UPV/EHU. Spain.

Shining light on cortico-cortical connections. Leopoldo Petreanu. (Invited speaker).

Label-free multimodal micro-spectroscopic differentiation of dysplasia stages in mouse tongues as a model of early tumor genesis in head and neck squamous cell carcinoma combined with multivariate data analysis. Mona Stefanakis.

Enhancement of second-harmonic microscopy images by combining adaptive optics and polarization modulation.Juanma Bueno.

Confocal microscopy and gene expression approaches to analyze auxin dynamics during microspore embryogenesis.Maria Carmen Risueño.

13:00 - 14:00 On-site equipment workshops (parallel x3)

Leica TCS-SP8x: flexible excitation and super resolution.

Andor Dragonfly: Spinning disk based multi-modality platform.

Zeiss Airyscan goes Fast: an innovative confocal imaging technology allows high resolution, high speed and low noise - simultaneously.

14:00 - 15:00 Buffet lunch
15:00 - 16:10 Session V: Functional Imaging: FRET. Invited and contributed talks

Chair: Jose Requejo. Instituto Biofisika. (CSIC, UPV/EHU).

Ascertaining HIV-1 entry and fusion in primary cells with FRET-based biosensors. Sergi Padilla-Parra. (Invited speaker).

Equilibrium parameters derived from FRET experiments in living cells. Álvaro Villarroel.

Molecular images of plitidepsin-eEF1A complexes in living tumor cells. Pilar Lillo.

16:10 - 16:40 Coffee break
16:40 - 17:40 Technical Workshop (parallel x2)

SPIM (Lightsheet microscopy) and OPT (Optical Projection Tomography) Workshop.

Deconvolution for digital microscopy Workshop.

17:40 - 18:40 Remoa Meeting

Chair: Maria Calvo. Universitat de Barcelona. Spain.

20:00 - 22:00 Gala Dinner
7Oct
Friday
08:30 - 9:00 Registration
09:00 - 11:00 Session VI: SPIM & in-vivo imaging. Invited talks

Chair: Gaby G. Martins. Instituto Gulbenkian de Ciência. Portugal.

Cellular dynamics in embryonic morphogenesis based on 3D+time imaging data. Nadine Peyrieras.

Imaging model diseases in transparent organs. Julien Colombelli.

3D Live Imaging of Fast Dynamic Processes. Jorge Ripoll.

11:00 - 11:30 Coffee
11:30 - 12:15 Session VI continued. Contributed talks

Chair: Julien Colombelli. IRB Barcelona. Spain.

Correction of attenuation artefacts in fluorescence microscopy. Jim Swoger.

Openspin and opent mesoscopic imaging for cell & developmental biology. Gabriel Martins.

High throughput three-dimensional cellular models imaging through flow light-sheet fluorescence microscopy cytometry. Emilio José Gualda.

12:15 - 12:55 New & notable. Invited talks

Chair: Nuno Moreno. Instituto Gulbenkian de Ciência, Portugal.

Fluorescent speckle microscopy by speckled photoswitching. António José Pereira.

12:55 - 13:35 Facility managing. Invited talks

Chair: Nuno Moreno. Instituto Gulbenkian de Ciência, Portugal.

Running a Core Facilities programme: Strategic planning to facilitate research. Monica Morales.

13:35 - 13:50 EuBI / EuBI Spain. Invited talks

EuBI / EuBI Spain. Timo Zimmermann

13:50 - 14:00 Closing remarks