This module includes the structural gene for the antibiotic of choice with its own native promoter. This segment is flanked by unique SwaI and PshAI restriction sites.
Available antibiotic markers:
Origin of replication
This module comprises the oriV sequence and the replication proteins, and it is flanked by the FseI and AscI restriction sites.
Available replication origins:
This DNA portion confers functionality to the vectors. This region is formatted as a DNA segment flanked by PacI and SpeI sites.
Available cargo types:
- Cloning (MCS, lacZ-alpha and lacZ-alpha-I-SceI)
- Expression systems (lacIq-Ptrc and xylS-Pm)
- Promoter-probe (lacZ, luxCDABE, YFP, CFP, DsRed2, and mCherry)
Gadgets are DNA sequences that endow a new functionality or property to the basic frame of the SEVA plasmids. The extant structure of the vectors allow addition of up to 6 new gadgets of the kind, as this is the number of unusual (and unique) restriction sites that punctuate the intervening regions between the 3 primary modules. Such sites are in principle permissive for insertions of such functional sequences, although only one of them (SanDI-SwaI) has been used thus far.