pSEVA-sib collection

The pSEVA-sib collection. This is a series of plasmids contributed by the extended community of SEVA users in which most features of the standard are kept but some others diverge from the canon. Contributors kindly offered their constructs for sharing through the SEVA database and plasmid repository.

 

Name Resistance ORI Cargo-like Gadget GenBank # or seq LabGenius view Developer Reference
pS234G Km pBBR1 lacIq-Ptrc-GFP pS234G.gbk de Lorenzo´s lab
pSEVA237R_Pem7 Km pBBR1 PEM7-mcherry pSEVA237R_Pem7 de Lorenzo´s lab
pSEVAT1Z1A Ap R6K thyA complement, Zeta toxin KJ686596 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAD1Z1A Ap R6K dapA complement, Zeta toxin KJ686597 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAT1K1A Ap R6K thyA complement, Kid toxin KJ686598 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAD1K1A Ap R6K dapA complement, Kid toxin KJ686599 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAT7Z1A Ap ColE2-P9 thyA complement, Zeta toxin KJ686600 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAD7Z1A Ap ColE2-P9 dapA complement, Zeta toxin KJ686601 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAT7K1A Ap ColE2-P9 thyA complement, Kid toxin KJ686602 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVAD7K1A Ap ColE2-P9 dapA complement, Kid toxin KJ686603 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA12DKR-A Km RK2 dapA targeting homology arms, pi-A rep gene, Kis antitoxin, FRT-KanR-FRT KJ686604 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA12DKR-E Km RK2 dapA targeting homology arms, pi-E rep gene, Kis antitoxin, FRT-KanR-FRT KJ686605 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA12DKR-J Km RK2 dapA targeting homology arms, pi-J rep gene, Kis antitoxin, FRT-KanR-FRT KJ686606 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA12TEC-B Km RK2 thyA targeting homology arms, repA-B rep gene, Epsilon antitoxin, FRT-KanR-FRT KJ686607 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA12TEC-I Km RK2 thyA targeting homology arms, repA-I rep gene, Epsilon antitoxin, FRT-KanR-FRT KJ686608 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA12TEC-K Km RK2 thyA targeting homology arms, repA-J rep gene, Epsilon antitoxin, FRT-KanR-FRT KJ686609 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA3b61 Cm  pUC-pTHT15 Shuttle vector for use in E. coli or B. subtilis KJ686616 Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pSEVA3b67Rb Cm  pUC-pTHT15 Shuttle vector with constitutive mRFP1,
compatible as reporter for E. coli/B. Subtilis
Ellis´lab Wright O. et al. ACS
Synthetic Biology. 2014.
pBAMD1-2 Ap, Km R6K mini-Tn5 transposon KM403113 de Lorenzo´s lab Martínez-García E. et al. Frontiers in Bioengineering and Biotechnology. 2014.
pBAMD1-4 Ap, Sm/Sp R6K mini-Tn5 transposon KM403114 de Lorenzo´s lab Martínez-García E. et al. Frontiers in Bioengineering and Biotechnology. 2014.
pBAMD1-6 Ap, Gm R6K mini-Tn5 transposon KM403115 de Lorenzo´s lab Martínez-García E. et al. Frontiers in Bioengineering and Biotechnology. 2014.
pBG28 Gm, Km R6K mini-Tn7 transposon, translational coupler , synthetic promoter 14a KR872418 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pTn7-M Gm, Km R6K mini-Tn7 transposon, MCS KR920750 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG37 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14c KT192132 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pBG51 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14d KT192133 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pBG42 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14g KT192134 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pM13 Gm, Km R6K mini-Tn7 transposon, SEVA cargo: 13 KT192135 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG Gm, Km R6K mini-Tn7 transposon, translational coupler KT192136 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG13 Gm, Km R6K mini-Tn7 transposon, translational coupler, SEVA cargo: 13 KT192137 de Lorenzo´s lab Zobel et al., ACS Synthetic Biology. 2015
pBG17 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14e KT192138 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pBG25 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14f KT192139 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pBG35 Gm, Km R6K mini-Tn7 transposon, translational coupler, synthetic promoter 14b KT192141 Blank’s Lab Zobel et al., ACS Synthetic Biology. 2015
pSCM Km pBBR1 chnR-PchnB –> msfGFPP pSEVA2311-msfGFP de Lorenzo´s lab