Nomenclature 2.0. The large number of SEVA constructs that have been generated with novel origins of replication and cargos has encouraged us to adopt an extended code for the vector collection. As shown in the scheme below, we consider each construct to be composed of 4 functional modules (antibiotic resistance marker, replication origin, cargo and gadget) which correspond to 4 inequivocal positions in a cipher. Each position can have one or more alphanumeric symbols as follows.
The first 9 antibiotic resistance markers receive a sole numeric code 1-9. Beyond that number, a capital letter will be added to ‘9’, starting with an A (9A) and following with 9B, 9C etc. It is unlikely that the number of new antibiotic resistances will exceed the capacity of such enumeration.
The first 9 origins of replication receive a sole numeric code 1-9. Beyond that number, a capital letter will be added to ‘9’, starting with an A (9A) and following with 9B, 9C etc. It is unlikely that the number of new origins of replication will exceed the capacity of such enumeration.
Each cargo is assigned a sole ordinal numeric code 1 to n (n being unlimited). This is because the number of cargoes will surely grow much faster than the other functional modules and thus this position of the code is intended not to be restrained.
Gadgets are designated by lower case greek letters (alpha to omega). This gives 24 possibilities, which, in case of completion can similarly be extended with capital greek letters (not to be expected anytime soon).
Example: a pSEVA code 9C-9E-13-α (abridged: 9C9E13α) means a vector with a 9C antibiotic resistance marker, a 9E origin of replication, bearing a cargo #13 and endowed with a gadget.