CNB Histology Facility
Centro Nacional de Biotecnología (CNB) Consejo Superior de Investigaciones Científicas (CSIC) Campus de Excelencia UAM+CSIC

 

telephone: 915854903
histologia@cnb.csic.es

 

CNB Histology Facility

 

Time-table for contacting/receiving/delivering samples:

from 08:00 to 15:00 hours

 

Centro Nacional de Biotecnología

Departament of Molecular and Cellular Biology

(Laboratory 126)

Campus de Cantoblanco, C/ Darwin 3

28049 Madrid

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Responsible Scientist: Lluís Montoliu (tel. 915854844, Laboratory 111)
Technician Specialist: Soledad Montalbán (tel. 915855349-office 128; 915854903-Laboratory 126)

Supporting Technician: Oscar Sánchez (tel. 915854903-Laboratory 126; 915854530-office Laboratory 111)

The CNB Histology Facility is integrated within the Scientific-Technological Platform INNOTEK, in support of research,

belonging to the International Campus of Excellence UAM+CSIC.

 

CNB Histology Facility Report (2015-2016)

Presentation of CNB Histology Facility (8 March 2012)

Histology Facility (January 2017) - Photo: Inés Poveda

 
Wax/Paraffin                                                       Frozen (Cryostat)                      Plastic/resin (Historesin)

Our Forms

Our Expertise

You can browse this table to see the species, tissues and organs we have proccessed to date

last update for expertise-table:  January 2013

Our Fees

 

Last update for these fees: January 2015 (fees maintained since June 2013)

Important note for external clients/researchers (not CNB): We will not invoice for a value less than 100 Euros.

Smaller values belonging to the same researcher/client will be accumulated and invoiced collectively, upon reaching 100 Euros,

or at the end of the year, in case this lower limit has not been reached.

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For invocing services to entities external to CNB we kindly appreciate to fill and send this invocing form  

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Service
Unit

Fee per unit

(CNB)


Fee per unit 

(other CSIC centres)

Fee per unit
(other public research institutions, not CSIC)

+21 % VAT
not included 
 

Fee per unit
(private research institutions)

+21 % VAT
not included

Preparing a paraffin block
1 U = 1 block
 5,5 Euros 6,9 Euros 8,9 Euros 13,4 Euros
Cutting a paraffin block 1 U = 1 block / 10 slides 5,0 Euros 6,2 Euros 8,0 Euros 12,1 Euros
" 1 U = 1 block / 5 slides 3,3 Euros 4,1 Euros 5,4 Euros 8,0 Euros
Staining Hematoxyline/Eosine or Cresyl Violet and mounting (paraffin)
1 U = 10 slides
 5,0 Euros 6,2 Euros 8 Euros 12,1 Euros

"

1 U = 5 slides

 3,3 Euros 4,1 Euros 5,4 Euros 8,0 Euros
Preparing a frozen block (cryostat)
1 U = 1 block
2,2 Euros 2,8 Euros 3,6 Euros 5,4 Euros
Cutting a frozen block (cryostat)
1 U = 10 slides
 5,0 Euros 6,2 Euros 8,0 Euros 12,1 Euros

"

1 U = 5 slides

 3,3 Euros 4,1 Euros 5,4 Euros 8,0 Euros
Staining Hematoxyline/Eosine or Cresyl Violet and mounting (frozen)
1 U = 10 slides
2,8 Euros 3,4 Euros 4,5 Euros 6,7 Euros

"

1 U = 5 slides

 1,7 Euros 2,1 Euros 2,7 Euros 4,0 Euros
Preparing a plastic block (Historesin)
1 U = 1 block
 17,6 Euros 22,0 Euros 28,6 Euros 42,9 Euros
Cutting a plastic block (Historesin)
1 U = 10 slides
19,8 Euros 24,8 Euros 32,2 Euros 48,3 Euros

"

1 U = 5 slides

 16,5 Euros 20,6 Euros 26,8 Euros 40,2 Euros
Staining Hematoxyline/Eosine or Cresyl Violet and mounting (plastic)
1 U = 10 slides
1,7 Euros 2,1 Euros 2,7 Euros 4,0 Euros

"

1 U = 5 slides

 1,1 Euros 1,4 Euros 1,8 Euros 2,7 Euros
Staining Trichromic Masson and mounting
1 U = 10 slides
22,0 Euros 27,5 Euros 35,8 Euros 53,6 Euros

"

1 U = 5 slides

 11,0 Euros 13,8 Euros 17,9 Euros 26,8 Euros
Staining PAS and mounting
1 U = 10 slides
7,0 Euros 9,0 Euros 12,0 Euros 18,0 Euros

"

1 U = 5 slides

 3,5 Euros 4,5 Euros 6,0 Euros 9,0 Euros
Staining Van Gieson-Elastin and mounting
1 U = 10 slides
15,0 Euros 19,0 Euros 24,0 Euros 36,0 Euros

"

1 U = 5 slides

 7,5 Euros 9,5 Euros 12,0 Euros 18,0 Euros
Staining Sirius Red and mounting
1 U = 10 slides
15,0 Euros 19,0 Euros 24,0 Euros 36,0 Euros

"

1 U = 5 slides

 7,5 Euros 9,5 Euros 12,0 Euros 18,0 Euros
Other procedures 
(specific stains, immunohistochemistry, ....)
please inquire
please inquire please inquire please inquire  please inquire 

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Important remarks:
1) The requested services by CNB personnel will always have preference for execution before the rest of orders from external users.
2) Unless specifically indicated, all blocks will be prepared and fixed in 4% PFA.
3) Unless specifically indicated, all sections from paraffin and plastic blocks will be obtained at 5 micrometers thickness, and those from frozen blocks at 15 micrometers thickness.
4) We offer two routine basic staining protocols, initially available: Hematoxylin/Eosin or Cresyl Violet. For other staining procedures, please inquire.
5) If the user needs less slides than indicated in the contracted 1 unit (10 or 5 slides) we will invoice still 1 Unit.


Equipment available

1 motorized Microtome (plastic/resin/paraffin/wax) Leica RM2155

1 motorized Microtome (plastic/resin/paraffin/wax) Thermo Scientific, Shandon, FINESSE ME+

1 Tisue Processing Station Leica TP1020

1 Cryostat Leica CM1900

1 Tissue Embedding Station Leica EG1160

2 thermostatic baths Leica HI1210


Coordination

Since 2009, the CNB Histology Facility is associated with the IIBm-UAM/CSIC Histology Facility. The two centres, participating in INNOTEK, merged their operations under the coordination of the CNB Histology Facility, enabling them to offer CNB and IIB researchers greater processing capacity of histological samples. The CNB Histology Facility has been training personnel from the IIB-UAM/CSIC Histology Facility and supervising work, aiming to establish equivalent output standards.


Publications

Florian Chevalier, Soledad Montalbán Iglesias, Óscar Javier Sánchez, Lluís Montoliu and Pilar Cubas (2014) Plastic Embedding of Arabidopsis Stem Sections. Bio-protocol 4(20): e1261


WEB last updated on: 08 February 2018