PROTOCOLS of the Mouse Embryo Cryopreservation Facility of CNB

Essentially, at the Mouse Embryo Cryopreservation Facility of CNB, we use the ultrarapid method of cryopreservation, which uses DMSO and 8-cell embryos, from an original protocol described by Jillian Shaw and later adapted by Kristina Vintersten.

Protocol for freezing and thawing mouse 8-cell embryos

For Sperm Cryopreservation we use the method originally described by Peter Glenister

Protocol for freezing mouse sperm and for in vitro fertilization

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