CNB Histology Facility

telephone: 915854903
histologia@cnb.csic.es

Time-table for contacting/receiving/delivering samples:

from 08:00 to 15:00 hours

Centro Nacional de Biotecnología

Departament of Molecular and Cellular Biology

(Laboratory 126 and Office 128)

Campus de Cantoblanco, C/ Darwin 3

28049 Madrid

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Responsible Scientist: Lluís Montoliu (tel. 915854844, Laboratory 111)
Technician Specialist: Soledad Montalbán (tel. 915854903-office 128; 915854903-Laboratory 126)
Supporting Technician: Oscar Sánchez (tel. 915854903-office 128; 915854903-Laboratory 126)
 

The CNB Histology Facility is integrated within the Scientific-Technological Platform INNOTEK, in support of research,

belonging to the International Campus of Excellence UAM+CSIC.

CNB Histology Facility Report (2017-2018)

Presentation of CNB Histology Facility (8 March 2012)

Personnel of Histology Facility (June 2022) - Photo: Lluís Montoliu

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Office 128 and Laboratory 126 at CNB: working during the pandemic with protection and safety distances. Photos (October 2020): Lluís Montoliu

 
Wax/Paraffin                                                       Frozen (Cryostat)                      Plastic/resin (Historesin)

Our Forms

WEB FORM FOR REQUESTING SERVICES

Our Expertise

You can browse this table to see the species, tissues and organs we have proccessed to date

last update for expertise-table:  February 2020

New Official Fees CSIC: 1st April 2024

 

For invocing services to entities external to CNB we kindly appreciate to fill and send this invocing form  

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Service	Unit	Fee per unit (CNB) (@) Non-EU projects €	Fee per unit  (other CSIC centres) (@) Non-EU projects  €	Fee per unit  (*)European projects CNB & CSIC   €	Fee per unit (other public research institutions, not CSIC) € +21 % VAT not included	Fee per unit (private research institutions) € +21 % VAT not included
Preparing a paraffin block 1 block	1 U = 1 block	4,47	5,24	10,35	13,42	14,69
Cutting a paraffin block 10 slides	1 U = 1 block / 10 slides	4,51	5,29	13,92	18,04	19,76
Cutting a paraffin block 5 slides	1 U = 1 block / 5 slides	2,26	2,65	6,96	9,02	9,88
Stain and mount paraffin with HE 10 slides	1 U = 10 slides	5,51	6,46	20,20	26,20	28,69
Stain and mount paraffin with HE 5 slides	1 U = 5 slides	2,74	3,22	17,44	22,61	24,77
Stain and mount paraffin with cresyl violet 10 slides	1 U = 10 slides	5,51	6,46	20,20	26,20	28,69
Stain and mount paraffin with cresyl violet 5 slides	1 U = 5 slides	2,74	3,22	17,44	22,61	24,77
Preparing a frozen block 1 block	1 U = 1 block	1,81	2,12	7,69	9,97	10,92
Cutting a frozen block 10 slides	1 U = 10 slides	4,51	5,29	19,21	24,90	27,28
Cutting a frozen block 5 slides	1 U = 5 slides	2,26	2,65	9,31	12,07	13,22
Stain and mount frozen with HE 10 slides	1 U = 10 slides	3,63	4,26	18,33	23,77	26,03
Stain and mount frozen with HE 5 slides	1 U = 5 slides	1,81	2,12	16,51	21,40	23,44
Stain and mount frozen with cresyl violet 10 slides	1 U = 10 slides	3,63	4,26	18,33	23,77	26,03
Stain and mount frozen with cresyl violet 5 slides	1 U = 5 slides	1,81	2,12	16,51	21,40	23,44
Preparing a plastic block 1 block	1 U = 1 block	12,92	15,15	21,73	28,18	30,87
Cutting a plastic block 10 slides	1 U = 10 slides	15,50	18,18	39,01	50,59	55,41
Cutting a plastic block 5 slides	1 U = 5 slides	7,75	9,09	19,51	25,29	27,70
Stain and mount plastic with HE 10 slides	1 U = 10 slides	2,28	2,67	16,97	22,01	24,10
Stain and mount plastic with HE 5 slides	1 U = 5 slides	1,13	1,33	15,83	20,52	22,48
Stain and mount plastic with CV 10 slides	1 U = 10 slides	2,28	2,67	16,97	22,01	24,10
Stain and mount plastic with CV 5 slides	1 U = 5 slides	1,13	1,33	15,83	20,52	22,48
Stain and mount paraffin/frozen with TRICR. MASSON 10 slides	1 U = 10 slides	23,31	27,35	38,01	49,29	53,98
Stain and mount paraffin/frozen with TRICR. MASSON 5 slides	1 U = 5 slides	11,66	13,67	26,35	34,17	37,43
Stain and mount paraffin/frozen with PAS 10 slides	1 U = 10 slides	6,49	7,61	21,19	27,47	30,09
Stain and mount paraffin/frozen with PAS 5 slides	1 U = 5 slides	3,24	3,80	17,93	23,26	25,47
Stain and mount paraffin with VAN GIESON ELASTIN 10 slides	1 U = 10 slides	12,84	15,07	27,54	35,71	39,11
Stain and mount paraffin VAN GIESON ELASTIN 5 slides	1 U = 5 slides	6,42	7,53	21,11	27,38	29,98
Stain and mount paraffin SIRIUS RED 10 slides	1 U = 10 slides	11,44	13,41	26,13	33,88	37,11
Stain and mount paraffin SIRIUS RED 5 slides	1 U = 5 slides	5,72	6,71	20,41	26,47	28,99
Other procedures  (specific stains, immunohistochemistry, ....)	please inquire	please inquire	please inquire	please inquire	please inquire	please inquire

(@) Fees for CNB and other CSIC centres, when using funds from non-EU projects to pay the requested service.

(*) Fees for CNB and other CSIC centres, when using funds from EU-Prohects (Horizon Europe/ERC...) ro pay the requested service.

HE stands for hematoxylin/eosine staining, CV stands for cresyl violet staining

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WEB FORM FOR REQUESTING SERVICES

Important remarks:
1) The requested services by CNB personnel will always have preference for execution before the rest of orders from external users.
2) Unless specifically indicated, all blocks will be prepared and fixed in 4% PFA.
3) Unless specifically indicated, all sections from paraffin and plastic blocks will be obtained at 5 micrometers thickness, and those from frozen blocks at 15 micrometers thickness.
4) We offer two routine basic staining protocols, initially available: Hematoxylin/Eosin or Cresyl Violet. For other staining procedures, please inquire.
5) If the user needs less slides than indicated in the contracted 1 unit (10 or 5 slides) we will invoice still 1 Unit.

Equipment available

1 motorized Microtome (plastic/resin/paraffin/wax) Leica RM2155	1 motorized Microtome (plastic/resin/paraffin/wax) Thermo Scientific, Shandon, FINESSE ME+	1 Tissue Processing Station Leica PEARL
1 Cryostat Leica CM1950	1 Tissue Embedding Station Leica EG1160	2 thermostatic baths Leica HI1210
1 stereo microscope Leica M80	1 Optical Microscope Leica DM4-B	1 drying oven Raypa

Coordination

Since 2009, the CNB Histology Facility is associated with the IIBm-UAM/CSIC Histology Facility. The two centres, participating in INNOTEK, merged their operations under the coordination of the CNB Histology Facility, enabling them to offer CNB and IIB researchers greater processing capacity of histological samples. The CNB Histology Facility has been training personnel from the IIB-UAM/CSIC Histology Facility and supervising work, aiming to establish equivalent output standards.

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Publications

Florian Chevalier, Soledad Montalbán Iglesias, Óscar Javier Sánchez, Lluís Montoliu and Pilar Cubas (2014) Plastic Embedding of Arabidopsis Stem Sections. Bio-protocol 4(20): e1261

WEB last updated on: 17 April 2024