CNB Histology Facility
Centro Nacional de Biotecnología (CNB) Consejo Superior de Investigaciones Científicas (CSIC) Campus de Excelencia UAM+CSIC

 

telephone: 915854903
histologia@cnb.csic.es

 

CNB Histology Facility

 

Time-table for contacting/receiving/delivering samples:

from 08:00 to 15:00 hours

 

Centro Nacional de Biotecnología

Departament of Molecular and Cellular Biology

(Laboratory 126 and Office 128)

Campus de Cantoblanco, C/ Darwin 3

28049 Madrid

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Responsible Scientist: Lluís Montoliu (tel. 915854844, Laboratory 111)
Technician Specialist: Soledad Montalbán (tel. 915854903-office 128; 915854903-Laboratory 126)

Supporting Technician: Oscar Sánchez (tel. 915854903-office 128; 915854903-Laboratory 126)
Supporting Technician CIBER-ISCIII: Inés Arroba (tel. 915854530-Laboratory 111)

The CNB Histology Facility is integrated within the Scientific-Technological Platform INNOTEK, in support of research,

belonging to the International Campus of Excellence UAM+CSIC.

 

CNB Histology Facility Report (2017-2018)

Presentation of CNB Histology Facility (8 March 2012)

 

Personnel of Histology Facility (June 2022) - Photo: Lluís Montoliu

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Office 128 and Laboratory 126 at CNB: working during the pandemic with protection and safety distances. Photos (October 2020): Lluís Montoliu

Personnel of Histology Facility (June 2021) - Photo: Lluis Montoliu

 
Wax/Paraffin                                                       Frozen (Cryostat)                      Plastic/resin (Historesin)

Our Forms

Our Expertise

You can browse this table to see the species, tissues and organs we have proccessed to date

last update for expertise-table:  February 2020

New Official Fees CSIC: 1st April 2023

 

For invocing services to entities external to CNB we kindly appreciate to fill and send this invocing form  

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Service
Unit

Fee per unit

(CNB)


Fee per unit 

(other CSIC centres)

Fee per unit
(other public research institutions, not CSIC)

+21 % VAT
not included 
 

Fee per unit
(private research institutions)

+21 % VAT
not included

Preparing a paraffin block
1 U = 1 block
 5,47 6,42 14,49 15,87
Cutting a paraffin block 1 U = 1 block / 10 slides 5,54 6,49 19,00 20,81
" 1 U = 1 block / 5 slides 2,76 3,24 9,50 10,40
Staining Hematoxyline/Eosine or Cresyl Violet and mounting (paraffin)
1 U = 10 slides
 6,74 7,90 27,21 29,81

"

1 U = 5 slides

3,36 3,95 22,84 25,01
Preparing a frozen block (cryostat)
1 U = 1 block
2,23 2,61 10,28 11,25
Cutting a frozen block (cryostat)
1 U = 10 slides
 5,54 6,49 25,66 28,10

"

1 U = 5 slides

 2,76 3,24 12,45 13,64
Staining Hematoxyline/Eosine or Cresyl Violet and mounting (frozen)
1 U = 10 slides
4,45 5,22 24,25 26,56

"

1 U = 5 slides

 2,23 2,61 21,36 23,40
Preparing a plastic block (Historesin)
1 U = 1 block
 15,82 18,56 31,60 34,61
Cutting a plastic block (Historesin)
1 U = 10 slides
18,98 22,27 54,18 59,34

"

1 U = 5 slides

 9,49 11,13 27,09 29,67
Staining Hematoxyline/Eosine or Cresyl Violet and mounting (plastic)
1 U = 10 slides
2,79 3,28 22,10 24,20

"

1 U = 5 slides

 1,39 1,63 20,28 22,21
Staining Trichromic Masson and mounting
1 U = 10 slides
28,55 33,49 55,50 60,78

"

1 U = 5 slides

 14,27 16,74 36,98 40,50
Staining PAS and mounting
1 U = 10 slides
7,94 9,31 28,77 31,51

"

1 U = 5 slides

3,97 4,65 23,62 25,87
Staining Van Gieson-Elastin and mounting
1 U = 10 slides
15,73 18,46 38,88 42,58

"

1 U = 5 slides

7,87 9,23 28,68 31,41
Staining Sirius Red and mounting
1 U = 10 slides
 14,01 16,43 36,64 40,13

"

1 U = 5 slides

 7,00 8,21 27,56 30,18
Other procedures 
(specific stains, immunohistochemistry, ....)
please inquire
please inquire please inquire please inquire  please inquire 

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Important remarks:
1) The requested services by CNB personnel will always have preference for execution before the rest of orders from external users.
2) Unless specifically indicated, all blocks will be prepared and fixed in 4% PFA.
3) Unless specifically indicated, all sections from paraffin and plastic blocks will be obtained at 5 micrometers thickness, and those from frozen blocks at 15 micrometers thickness.
4) We offer two routine basic staining protocols, initially available: Hematoxylin/Eosin or Cresyl Violet. For other staining procedures, please inquire.
5) If the user needs less slides than indicated in the contracted 1 unit (10 or 5 slides) we will invoice still 1 Unit.


Equipment available

1 motorized Microtome (plastic/resin/paraffin/wax) Leica RM2155

1 motorized Microtome (plastic/resin/paraffin/wax) Thermo Scientific, Shandon, FINESSE ME+

1 Tissue Processing Station Leica PEARL

1 Cryostat Leica CM1950

1 Tissue Embedding Station Leica EG1160

2 thermostatic baths Leica HI1210

1 stereo microscope Leica M80

1 Optical Microscope Leica DM4-B

1 drying oven Raypa


Coordination

Since 2009, the CNB Histology Facility is associated with the IIBm-UAM/CSIC Histology Facility. The two centres, participating in INNOTEK, merged their operations under the coordination of the CNB Histology Facility, enabling them to offer CNB and IIB researchers greater processing capacity of histological samples. The CNB Histology Facility has been training personnel from the IIB-UAM/CSIC Histology Facility and supervising work, aiming to establish equivalent output standards.


Publications

Florian Chevalier, Soledad Montalbán Iglesias, Óscar Javier Sánchez, Lluís Montoliu and Pilar Cubas (2014) Plastic Embedding of Arabidopsis Stem Sections. Bio-protocol 4(20): e1261


Acknowledgements in publications

Sierra-Ramos C, Velazquez-Garcia S, Vastola-Mascolo A, Hernández G, Faresse N, Alvarez de la Rosa D.
SGK1 activation exacerbates diet-induced obesity, metabolic syndrome and hypertension
J Endocrinol. 2020 Jan;244(1):149-162.

Seruggia D, Fernández A, Cantero M, Fernández-Miñán A, Gomez-Skarmeta JL, Pelczar P, Montoliu L.
Boundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression
Sci Rep. 2020 Sep 23;10(1):15494.

Murillo-Cuesta S, Celaya AM, Cervantes B, Bermúdez-Muñoz JM, Rodríguez-de la Rosa L, Contreras J, Sánchez-Pérez I, Varela-Nieto I.
Therapeutic efficiency of the APAF-1 antagonist LPT99 in a rat model of cisplatin-induced hearing loss
Clin Transl Med. 2021 Apr;11(4):e363.

Gil-Ranedo J, Gallego-García C, Almendral JM.
Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations
Cell Rep. 2021 Sep 7;36(10):109673.

Pérez P, Lázaro-Frías A, Zamora C, Sánchez-Cordón PJ, Astorgano D, Luczkowiak J, Delgado R, Casasnovas JM, Esteban M, García-Arriaza J.
A Single Dose of an MVA Vaccine Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Protein Neutralizes Variants of Concern and Protects Mice From a Lethal SARS-CoV-2 Infection
Front Immunol. 2022 Jan 27;12:824728.

Lázaro-Frías A, Pérez P, Zamora C, Sánchez-Cordón PJ, Guzmán M, Luczkowiak J, Delgado R, Casasnovas JM, Esteban M, García-Arriaza J.
 Full efficacy and long-term immunogenicity induced by the SARS-CoV-2 vaccine candidate MVA-CoV2-S in mice
NPJ Vaccines. 2022 Feb 9;7(1):17.

Borroto A, Alarcón B, Navarro MN.
Mutation of the Polyproline Sequence in CD3ε Evidences TCR Signaling Requirements for Differentiation and Function of Pro-Inflammatory Tγδ17 Cells
Front Immunol. 2022 Mar 31;13:799919.

Guardia A, Fernández A, Seruggia D, Chotard V, Sánchez-Castillo C, Kutsyr O, Sánchez-Sáez X, Zurita E, Cantero M, Rebsam A, Cuenca N, Montoliu L.
A Slc38a8 Mouse Model of FHONDA Syndrome Faithfully Recapitulates the Visual Deficits of Albinism Without Pigmentation Defects
Invest Ophthalmol Vis Sci. 2023 Oct 3;64(13):32.

Gonzalo-Gobernado R, Moreno-Martínez L, González P, Dopazo XM, Calvo AC, Pidal-Ladrón de Guevara I, Seisdedos E, Díaz-Muñoz R, Mellström B, Osta R, et al.
Repaglinide Induces ATF6 Processing and Neuroprotection in Transgenic SOD1G93A Mice
 International Journal of Molecular Sciences. 2023; 24(21):15783. 

 


WEB last updated on: 31 October 2023